摘要
目的初步探究鸡产蛋下降综合征病毒NE4(EDSV NE4)毒株对昆明小鼠的感染特性。方法用106TCID50攻毒量的EDSV NE4株对4～6周龄的KM小鼠进行攻毒试验,同时以正常尿囊液接种作为阴性对照。用荧光定量PCR对小鼠组织及粪便中的EDSV进行检测,同时用HE染色及免疫组化法对小鼠组织切片进行病理组织学观察和抗原定位。结果 EDSV攻毒对小鼠的采食情况产生一定影响,但并未引起明显的临床症状;攻毒组小鼠可产生针对EDSV特异性的抗体,HI抗体滴度可高达212,阴性对照组小鼠体内抗体检测为阴性;攻毒后小鼠大部分组织器官如肝脏、子宫、肾脏、肺脏等与攻毒7 d后粪便中均可检测到EDSV,阴性对照小鼠所有受检组织中均未检测到病毒;EDSV攻毒未能引起小鼠组织的病理学变化,攻毒后不同时间内,可在子宫、肺脏、肝脏、肾脏中可检测到阳性信号,最为典型的定殖位置是子宫腺体上皮细胞及肌层细胞的胞质中。结论 EDSV NE4株可以感染KM小鼠。
Objective To study the characteristics of mice infected with egg drop syndrome virus （EDSV） strain NE4. Methods Kunming （KM） mice in age of 4-6 weeks were artificially infected with 106 TCIDs0 dose of EDSV strain NE4 , and the negative control mice were inoculated with normal allantoic liquid at the same time. FQ-PCR assay was used to detect the quantity of virus in different tissues. HE and immunohistoehemical staining were used to observe the pathologi- cal changes and antigen localization. Results The virus inoculation affected somehow the ingestion of mice, but did not cause obvious clinical symptoms. The virus-infected mice produced specific antibody, reaching up to a titer of 212. The EDSV titer in the control mice was negative. EDSV was detected in the tissues of most organs such as liver, uterus, kid- ney, lungs of the mice by FQ-PCR assay, and at 7 days post infection, the mice began to release virus. Immunohistochem- istry showed that EDSV was propagated in organs such as liver, uterus, kidney, and lungs of the mice, most notably, in the cytoplasm of uterine glandular epithelial ceils and myocytes. No obvious pathological changes were found in tissues of infected mice. Conclusion EDSV strain NE4 virus can infect KM mice.
出处
《中国实验动物学报》
CAS
CSCD
2012年第6期43-46,I0006,I0007,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金(No.31172342)
国家公益行业项目(No.200903037-2)
国家高技术研究发展计划(863)项目(2008AA10Z411)
FAO/IAEA项目(14515)
关键词
鸡产蛋下降综合征病毒
NE4株
小鼠
感染特性
Egg drop syndrome virus （EDSV）
Strain NE4
Kunmimg mice
Characteristics
infection
