摘要
粘连细胞的分割是细胞精确计数的关键,针对牛乳粘连体细胞的特点,首先利用k-means方法分割细胞和背景并运用数学形态学算子对图像做预处理。然后对分割后的二值图像进行距离变换和图像重构,最后用Water-shed算法分离粘连细胞,以得到最终结果。实验结果表明该方法能准确、快速地分割粘连牛乳体细胞,并能有效改善分水岭(Watershed)的过分割现象。
The segmentation of overlapping cells is the key of accurate cell counting.According to the characteristics of milk somatic cell,the cells and background are segmented using the k-means method and the result image are preprocessed by Mathematical morphology operators.And then,the segmented binary images are processed by distance transform and image reconstruction.Finally,overlapping cells are separated by Watershed method.The experimental results show that the method can segment overlapping milk somatic cells accurately and quickly,and can also effectively resolve the problem of watershed over-segmentation.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2012年第3期222-225,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古农业大学科技创新团队项目(ZN201010)
教育部"春晖计划"项目(Z2009-1-01062)
