摘要
目的:探讨雌激素受体β1(ERβ1)在结直肠腺癌及癌旁正常组织中的表达及其临床意义。方法:收集结直肠腺癌石蜡标本91例,制作石蜡组织芯片,并以周围正常肠黏膜组织作对照,行免疫组织化学染色,分析其ERβ1、ERα、雄激素受体(AR)、孕激素受体(PR)表达情况及临床病理联系。结果:91例结直肠腺癌组织中,ERβ1阳性率为46.15%(42/91),周围正常肠黏膜ERβ1阳性率为62.64%(57/91),两者差异有统计学意义(P=0.018);结直肠腺癌中ERβ1表达情况与患者性别有关,男性(61.54%)高于女性(34.62%)(P=0.01),而与年龄、浸润深度、临床分期、肿瘤的分化程度、淋巴结转移,是否复发无关。正常大肠黏膜中ERβ1的阳性表达率男性(74.35%)高于女性(53.85%),差异有统计学意义(P=0.036)。ERα、AR、PR在结直肠腺癌及周围正常肠黏膜中阴性表达。结论:结直肠腺癌及癌旁正常黏膜都可表达ERβ1,ERβ1的失表达可能参与了结直肠腺癌的形成过程。
Objective： To probe the expression of estrogen receptor β1 （ER B 1） in colorectal adenocarci-noma and their clinical significance. Methods： Paraffin-embedded tissue of 91 cases of colorectal adenocarcinoma were collected and tissue microarray was prepared. Normal colorectal mucosa near to colorectal carcinoma was used as control. Expression of ER I5 1 was detected by immunohistochemical staining and the clinicopathological significance was analyzed. Results： The expression rates of ER β1 in colorectal adenocarcinoma and normal colorectal mucosa were 46.15% （42/91） and 62.64% （57/91） （P=0.018） respectively. ER 151 expression in male patient （61.54%） was significantly higher than that in female （34.62%） （P=0.01）, and there was no correlation between ER β1 expression with patients＇age, the depth of tumor invasion, clinical stages, histological grading, lymph node metastasis, and remission. The expression of ERβ1 in normal colorectal mucosa of male patients （74.35%） was higher than those of female （53.85%）, （P=0.036）. The expression of ER151, AR and PR was negative in all cases. Conclusion： ER β 1 is expressed in colorectal adenocarcinoma and normal mucosa. The loss of ER β 1 expression maybe involved in contribute to the development of colorectal adenocarcinoma.
出处
《温州医学院学报》
CAS
2013年第1期41-44,共4页
Journal of Wenzhou Medical College
关键词
结直肠肿瘤
雌激素受体β1
组织芯片
免疫组织化学
colorectal adenocarcinoma
estrogen receptor β1
tissue microarray
immunohistochemistry
