摘要
目的:建立C57BL/6J小鼠肝癌模型,研究高滴度腺病毒介导的蛋白酪氨酸磷酸酶1B(protein tyro-sine phosphatase 1B,PTP1B)在小鼠肝癌中的表达情况。方法:应用二乙基亚硝胺(diethylnitrosamine,DEN)/四氯化碳(carbon tetrachloride,CCl4)/乙醇诱导小鼠成癌,应用ELISA的方法检测小鼠血清中甲胎蛋白(al-phafetoprotein,AFP)水平,HE染色的方法镜下判断肝癌组织的形成,免疫组化的方法检测PTP1B在肝癌组织中的表达情况。结果:成功地建立了小鼠肝癌模型;肝癌组及正常对照组小鼠血清中AFP水平分别为(799.5±186.3)μg/L、(8.9±1.8)μg/L,两组相比较,差异有统计学意义(P<0.05);PTP1B在肝癌组及正常对照组中的表达水平分别92.16%和86.67%,两组相比较,差异无统计学意义(P>0.05)。结论:采用DEN 75mg/kg比100mg/kg腹腔注射联合应用CCl4/乙醇可以更好地建立肝癌小鼠模型;通过小鼠眼眶静脉内采血测定AFP来评估建模是否成功是可行的;PTP1B能够在肝癌组织中过量表达,其将为进一步实验研究奠定基础。
Objective：To investigate the feasibility of establishing the hepatocarcinoma model in C57BL/6J mice, and study the expression of protein tyrosine phosphatase 1B （PTP1 B） in tumor tissue. Methods：Liver tumors were induced by diethylnitrosamine （DEN）, carbon tetrachloride （CC14 ） and ethanol, the level of AFP was tested with en- zyme - linked immunosorbent assay （ELISA） method, and the liver tumors were examined by hematoxylin - eosin （HE） staining,then the expression of PTP1B in tumor tissue was investigated by means of immunohistochemical SP method. Results ：The models of hepatocarcinoma in C57BL/6J mice were established successfully. The level of AFP was（799.5 -＋ 186.3 ）ixg/L in hepatocarcinoma group and （8.9 -＋ 1.8 ）txg/L in control group, which reached hepato- carcinoma model of mice was established by intraperitoneal injection with DEN 75 mg/kg than 100 mg/kg. Conclu- sion ： AFP can be tested in orbital vein blood if the model successfully established, overexpression of PTP1B in hepato- carcinoma is detected, that will be the foundation for further experimental research.
出处
《现代肿瘤医学》
CAS
2013年第1期10-13,共4页
Journal of Modern Oncology
基金
国家自然科学基金资助项目(编号:30870950)
