摘要
以波兹南农业大学植物遗传育种系提供的甘蓝型胞质雄性不育油菜为母本与青花菜杂交 ,授粉后 1 9d,观察其种间杂交的有效性 ,并对其胚和胚珠进行离体培养。结果表明 :种间杂交的亲和性及胚珠退化时间随杂交组合不同而异 ,亲本的基因型对其有很大的影响。
Cytoplasm male sterility Brassica napus supplied by the Department of Genetics and Plant Breeding of Poznan Agricultural University was used as maternal plant to cross with Brassica oleracea var. italica . The interspecific cross effectiveness was observed, embryos and ovules were in vitro cultured at 19 days after pollinating. The results showed that the interspecific cross compatibility and the time of ovule degeneration varied with cross combinations, the genetype had a great influence on crossing. Embryo and ovule in vitro cultures could overcome postzygotic barriers effectively and obtained interspecific hybrids.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2000年第3期274-278,共5页
Journal of Huazhong Agricultural University
关键词
甘蓝型油菜
青花菜
种间杂种
胚
胚珠
离体培养
Brassica napus
B. oleracea var. italica
interspecific hybrid
embryo and ovule in vitro cultures
