摘要
目的探讨甲磺酸加贝酯对大鼠Ⅲ度烧伤后炎性反应的影响。方法选取健康大白鼠60只,随机分为烧伤脓毒血症对照组(A组)、烧伤脓毒症实验组(B组)、未烧伤脓毒血症对照组(C组)和未烧伤脓毒症实验组(D组),每组15只。制作烧伤模型,并单次腹腔注射脂多糖(5μg/g),造成脓毒症。实验组分别于烧伤后的第1天开始给予甲磺酸加贝酯股静脉注射30 mg/kg,1次/d;对照组同期给予等量生理盐水,3 d后测定外周血清白细胞介素-1(IL-1)、IL-6、肿瘤坏死因子-α(TNF-α)表达水平及其肺、脾中嗜中性多形核粒细胞(PMN)表达情况。结果 IL-1、IL-6、TNF-α表达在A组高于B、C、D组(P<0.05),C组高于D组(P<0.05),B组低于D组(P<0.05);PMN在各组肺、脾中的表达存在着差异,A组高于B、C、D组(P<0.05),B组低于C、D组(P<0.05),C组高于D组(P<0.05)。结论甲磺酸加贝酯能够有效减轻Ⅲ度烧伤后炎性反应,从而降低致炎因子对组织、器官的损伤。
Objective To investigate the effect of gabexate mesylate on inflammatory reaction of severe burned rats.Methods Sixty health rats were selected and randomly divided into burns sepsis control group（group A）,burns sepsis experimental group（group B）,unburns sepsis control group（group C）,unburns sepsis experimental group（group D）,and 15 rats in each groups.Make burn model,and single intraperitoneal injection of LPS（5 μg/g）,cause sepsis.Give gabexate mesylate to experimental group（30 mg/kg,once a day） in the first day after burns and control group gives physiological saline.Three days late,setting-out IL-1,IL-6,TNF-α in peripheral blood and expression of PMN in lune and spleen.Results The expression of IL-1,IL-6,TNF-α in group A was higher than that in group B,C and D（P0.05）;And in group C,it was higher than that in group D（P0.05）;In group B,it was less than that in group D（P0.05）.The expression of PMN in group A was higher than that in group B,C and D（P0.05）;And in group B,it was less than that in group C and D（P0.05）;In group C,it was higher than that in group C（P0.05）.Conclusion Gabexate mesylate can reduce the burns inflammation reaction,in order to reduce the tissue damage by inflammation factors.
出处
《临床医学》
CAS
2012年第12期21-23,共3页
Clinical Medicine
