摘要
以正己烷为溶剂,提取烟草中的茄尼醇,在浸提工艺前,引入氨浸预处理技术来有效增强提取效果。综合运用组分分析、液质联用、扫描电镜等技术分析了氨浸预处理的必要性和氨浸预处理机制。结果表明,氨浸预处理可以有效脱除木质素,破坏烟草细胞壁结构,促进茄尼醇释放,并可对茄尼酯进行水解皂化生成游离态茄尼醇,同时溶解部分杂质,从而强化茄尼醇的提取效果,简化后续纯化过程。提取对比实验表明,对烟叶原料进行氨浸预处理后,提取平衡时间从3 h缩短至1.5 h,茄尼醇提取率从89.24%±1.54%提高到104.63%±2.44%,浸膏纯度从16.72%±0.6%提高到21.03%±0.6%,效果显著。该法可为其他天然产物的提取工艺开发提供新的思路和方法。
A pretreatment method of aqueous ammonia leaching was used before the extraction of solanesol from tobacco leaves. The results of component analysis of tobacco leaves demonstrate that the pretreatment is necessary, and HPLC, MS and SEM analyses indicate that the positive effect of pretreatment was significant. The ammonia leaching could dissolve lignin, destroy the cell wall of tobacco,release the solanesol, change the solanesyl ester into solanesol, and dissolve some of the impurities,thereby significantly enhancing the extraction of solanesol and simplifying the subsequent purification process. After ammonia pretreatment, the equilibrium time of extraction was shortened from 3 h to 1.5 h,the extraction yield increased from 89.24% ±1.54% to 104.63% ±2.44% ,and the purity of extract increased from 16.72%±0.6% to 21.03% ±0.6%.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2013年第1期32-35,50,共5页
Fine Chemicals
基金
国家自然科学基金(20976125)
天津市应用基础研究计划面上项目(10JCYBJC05100)~~
关键词
茄尼醇
氨浸预处理
扫描电镜
液质联用
催化与分离提纯技术
solanesol
ammonia leaching pretreatment
SEM
HPLC - MS
catalysis, separation and purification technology
