摘要
采用序批式移动床生物膜反应器(MBBR),研究了以硝酸盐为电子受体的反硝化聚磷菌(DPB)的选择和富集.结果表明,采用3个阶段进行选择和富集,DPB占全部聚磷菌(PAOs)比例约从11.77%提高到66.07%;第3阶段培养末期,COD和TP去除率平均值分别为68.78%和69.02%,缺氧所耗ρ(NO3--N)达到23.91 mg/L;对反应器中生物膜进行直接染色发现,在厌氧放磷阶段能观察到聚磷菌体内有大量聚-β-羟丁酸(PHB)出现,而在缺氧吸磷阶段则有大量聚磷颗粒(Poly-p)出现,故可尝试采用聚磷生物膜的直接染色方法观察聚磷微生物细胞内PHB和Poly-p颗粒的变化,来判断生物除磷过程及效果.
The selection and dominance of denitrifying phosphorus removing bacteria （DPB） , which was capable of utilizing nitrate as electron acceptor, was investigated in a laboratory-scale sequencing batch moving-bed biofilm reactor （MBBR）. The experimental results demonstrated that the proportion of DPB increased approximately from 11.77% to 66.07% of total phosphate accumulating organisms （PAOs） after three stages selection and dominance. In the end of the third cultivating period, average removal efficiencies of COD and TP were 68.78% and 69.02% , respectively, and a large quantity of nitrate, 23.91 mg/L, was removed in anoxic phase. It was found that a great deal of PHB about PAOs emerged in anaerobic phase, whereas a mass of Poly-p about PAOs arose in anoxic phase by biofilm dyeing method, so they could be taken as evidences to judge the process and efficiency of biological phosphorus removal.
出处
《北京工业大学学报》
CAS
CSCD
北大核心
2013年第1期92-97,共6页
Journal of Beijing University of Technology
基金
国家自然科学基金资助项目(40372113)
关键词
反硝化除磷
反硝化聚磷菌
聚磷菌
移动床生物膜反应器
denitrifying phosphorus removal
denitrifying phosphorus removing bacteria
phosphate accumulating organisms
moving-bed biofilm reactor
