摘要
通过荧光定量PCR方法,测定了喂饲富锗木耳菌丝体小鼠肠道内大肠杆菌和乳酸杆菌的16srDNA数量,探讨了富锗木耳菌丝体对小鼠肠道菌群的影响。实验结果表明,喂饲富锗木耳菌丝体小鼠肠道内大肠杆菌数量未见明显变化(p>0.05),乳酸杆菌数量显著增加(p<0.01),富锗木耳对肠道菌大肠杆菌无明显的调节作用,对乳酸杆菌的生长繁殖有促进作用。
The numbers of 16s rDNA of Escherichia coli and Lactobacillus in the intestine of mice fed with germanium - riched Auricularia Auricular mycelia was detemined and the influence of germanium - riched Auricularia auricular mycelia on intestinal flora was investigated by fluorogenic quantitative PCR. The results showed that：no significant change of the number of Escherichia coli in the intestine of mice fed with germanium -riched Auricularia auricular mycelia was observed （p 0.05 ） ,and the number of Lactobacillus significantly increased （ p 0 . 01 ） . The germanium - riched Auricularia auricular mycelia had no significant regulation effect on Escherichia coli,but had promotive effect on Lactobacillus multiplication.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第2期346-348,352,共4页
Science and Technology of Food Industry
关键词
木耳
锗
肠道菌群
16S
RDNA
荧光定量PCR
Auricularia auricula
germanium
intestinal microflora
16S rDNA
fluorogenic quantitative PCR
