摘要
目的探讨人血白蛋白治疗对大鼠脑缺血早期海马血管内皮生长因子(VEGF)及fam样酪氨酸激酶受体1(flt-1)表达的影响。方法雄性SD大鼠40只,采用大脑中动脉线栓法制备大鼠局灶性脑缺血再灌注模型,随机分为假手术组10只、生理盐水组15只和白蛋白组15只。采用溴甲酚绿法测定血清白蛋白水平,RT-PCR检测脑缺血再灌注后6、24、48h大鼠海马VEGF和flt-1mRNA表达,免疫组织化学和Western blot法检测脑缺血再灌注24h海马VEGF蛋白表达。结果与生理盐水组比较,白蛋白组大鼠神经功能缺损评分于脑缺血再灌注后24、48h明显降低(P<0.05),海马VEGF mRNA和flt-1mRNA表达于脑缺血再灌注后6、24h明显降低(P<0.05,P<0.01),海马VEGF蛋白表达于脑缺血再灌注后24h明显降低(P<0.05)。结论白蛋白治疗可下调脑缺血早期海马VEGF和flt-1mRNA表达,降低海马VEGF蛋白表达水平,改善神经功能缺损。
Objective To study the effect of human albumin therapy on expression of VEGF and fam-like tyrosine kinase（flt-1） in rat hippocampus following ischemia/reperfusion（I/R）. Methods A rat focal cerebral I/R model was established by middle cerebral artery occlusion. Forty male SD rats were randomly divided into sham-operation group（n= 10）, normal saline treatment group （n= 15） ,and albumin treatment group（n= 15）. Their serum albumin level was measured by bromcresol green assay. Expression level of VEGF and flt-1 mRNA in hippocampus of rats was measured at 6,24 and 48 h after cerebral I/R by RT PCR. VEGF protein expression level in hippocampus was measured at 24 h after cerebral I/R by immunohistochemistry and Western blot,respectively. Results The nerve dysfunction score was significantly lower at 6 and 24 h after cerebral I/ R, the expression level of VEGF and flt-1 mRNA was significantly lower at 24 and 48 h after cerebral I/R,and the expression level of VEGF protein was significantly lower at 24 h after cerebral I/R in albumin treatment group than in normal saline treatment group（P〈0.05,P〈0.01）. Conclusion Albumin therapy improves the nerve dysfunction of rats after cerebral I/R by down-regulating the expression of VEGF mRNA and protein and flt-1 mRNA in their hippocampi
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2013年第1期82-85,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
天津市应用基础及前沿技术研究计划(09JCYBJC11700)
