摘要
目的:分析湖北省汉族女性亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,MTHFR)C677T、A1298C及甲硫氨酸合成酶还原酶(methionine synthase reductase,MTRR)A66G基因多态性的分布特征。方法:采用横断面调查研究方法,以湖北省2 899例汉族女性为对象,采集口腔黏膜上皮细胞提取基因组DNA,采用Taqman-MGB技术进行MTHFR和MTRR基因多态性检测,统计分析基因多态性的分布特征并与已报道的其他地区的数据进行比较。结果:湖北省汉族女性的MTHFR 677TT纯合突变基因型频率为16.0%,高于海南省,低于山东省和河南省(P<0.01),与四川省的差异无统计学意义(P>0.05)。MTHFR 1298CC纯合突变基因型频率为4.5%,高于河南省,低于四川省和海南省(P<0.05),与山东省的差异无统计学意义(P>0.05)。MTRR 66GG纯合突变基因型频率为6.2%,低于海南省(P<0.01),与山东、河南和四川省的差异均无统计学意义(P>0.05)。结论:湖北省汉族女性有不同于其他地区的MTHFR和MTRR基因多态性分布特征。
Objective： To analyze the distribution characteristics of gene polymorphism of methylenetetrahydrofolate reductase （MTHFR） C677T, A1298C and methionine synthase reductase （MTRR） A66G among the Han women in Hubei province. Methods： A cross - sectional study was used to select 2 899 Han women from Hubei province as study objects, oral epithelial cell samples were collected to abstract genome DNA, Taqman - MGB technique was used to detect gene polymorphism of MTHFR and MTRR and analyze the distribution characteristics of gene polymorphism, then the results were compared with data reported in other areas in China. Results： The frequency of MTHFR 677CC homozygous mutation in Han women in Hubei province was 16. 0%, which was statistically significantly higher than that in Hainan province and statistically significantly lower than those in Shandong province and Hehan province （ P 〈 0.01 ）, compared with the re- sult in Sichuan province, there was no statistically significant difference （ P 〉 0.05 ） . The frequency of MTHFR 1298 CT homozygous muta- tion in Han women in Hubei province was 16. 0%, which was statistically significantly higher than that in Helaan province and statistically significantly lower than those in Sichuan province and Hainan province （ P 〈 0. 05 ） , compared with the result in Shandong province, there was no statistically significant difference （ P 〉 0.05 ） . The frequency of MTRR 66 GG in Han women in Hubei province was 6. 2% , which was statistically significantly lower than that in Hainan province （P 〈 0.01 ） , compared with the results in Shandong province, Hehan prov- ince, and Sichuan province, there was no statistically significant difference （ P 〉 0. 05 ） . Conclusion ： The distribution characteristics of gene polymorphism of MTHFR and MTRR in Han women in Hubei province were different from distribution characteristics in other areas in China.
出处
《中国妇幼保健》
CAS
北大核心
2013年第1期140-143,共4页
Maternal and Child Health Care of China
