摘要
The gene of VEGF 165 was subcloned into the P.pastoris secretive expression vector pHIL S1 and the recombinant expression plasmid pHIL S1 VEGF 165 was constructed.After transformation into yeast GS115,the positive transformants were obtained through phenotype selection and DNA Dot blotting.After induction by methanol,soluble dimer VEGF 165 were expressed and secreted into the culture supernatant with its expression occupying 47% of the total protein in the supernatant.Dot blot analysis showed that the expressed human VEGF 165 could bind to its receptors flt 1 and KDR.
The gene of VEGF 165 was subcloned into the P.pastoris secretive expression vector pHIL S1 and the recombinant expression plasmid pHIL S1 VEGF 165 was constructed.After transformation into yeast GS115,the positive transformants were obtained through phenotype selection and DNA Dot blotting.After induction by methanol,soluble dimer VEGF 165 were expressed and secreted into the culture supernatant with its expression occupying 47% of the total protein in the supernatant.Dot blot analysis showed that the expressed human VEGF 165 could bind to its receptors flt 1 and KDR.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第4期531-533,共3页
Chinese Journal of Biotechnology
基金
国家高技术研究发展计划生物技术领域资助项目!( 10 0 9 0 2 0 4)
