摘要
光学椭偏显微成像是一种新型超薄膜及表面显示技术 ,是研究生物分子与固体表面吸附以及生物分子之间相互作用的一种简单、快速和可靠的手段。它不仅能够大面积精确显示超薄膜的厚度分布 ,而且能够用于表面实时吸附的动力学研究。在抗原 抗体检测分析方面 ,它不需要像酶联免疫法、荧光免疫法和放射免疫法那样对待测物作标记 ,也不会对待测生物分子活性造成任何扰动和损伤 ,操作简单 ,费用低廉。另外 ,它还弥补了传统的椭偏法的不足之处 ,能够有效地区分非特异性吸附、脱吸附或表面污染带来的干扰。
Imaging Ellipsometry is an optical surface sensitive method for the investigation of various aspects of biomolecules adsorbing mainly at reflecting metal surfaces and silicon surface.It has advantages of high sensitivity to layer thickness,big area of view,high sampling speed,and high lateral resolution.It can be used for studying adsorption kinetics of biomolecules and detecting complex layer of antigen antibody when antibody (or antigen) combined with antigen (or antibody) coated on solid surfaces.Compared with other solid phase methods such as enzyme linked immunosorbent assay,immunofluorescence and radioimmunoassay,imaging ellipsometry has the advantage of not involving any labelling of reactants and it is a relatively inexpensive method and easy to handle.Compared with traditional ellipsometer,imaging ellipsometry shows an advantage of distinguishing both affinty and non specific binding in different area on the surfaces.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第4期429-432,共4页
Chinese Journal of Biotechnology
基金
KJ95 2 J1 2 0 4
