摘要
利用ＰＣＲ技术获得满江红鱼腥藻ｇｌｎＡ启动区，经克隆测序后构成谷氨酰胺合成酶基因启动于驱动的ＧＵＳ基因表达载体。用基因枪转化，将表达载体导入烟草中，在其叶片和茎中检测到ＧＵＳ活性，而在烟草根中未见表达。实验结果对真核生物与原核生物间基因表达调控、转录因子识别的研究以及构建实用载体具有一定价值。
The promoter of Anabaena aazollae glnA gene was obtained by PCR procedure. A binary vector containing the chimeric gene of glnA / GUS was constructed after cloning and sequencing. The expression vector was transformed into tobacco by microprojectile bombardment. 'The GUS activities were examined histochemically in leaves and stems of the transgenic tobacco, but not in its roots. The result was valuable both in the regulation of gene expression, the recognition of transcriptional factors between eukaryota and prokaryota and the construction of practical vectors.
出处
《水生生物学报》
CAS
CSCD
北大核心
2000年第4期369-373,共5页
Acta Hydrobiologica Sinica
基金
国家自然科学基金!(39270414)
