摘要
目的 :了解鼠疫耶尔森氏菌 16S - 2 3SrRNA基因间区的酶切位点分布情况。方法 :PCR扩增及限制性内切酶分析。结果 :对EV菌及不同来源的 10 3株鼠疫耶尔森氏菌 16S - 2 3SrRNA基因间区进行了扩增 ,选用限制性内切酶TaqⅠ及MspⅠ对扩增产物进行酶切分析 ,结果发现所用鼠疫耶尔森氏菌该间区扩增产物及酶切图谱一致 ;选用TaqⅠ +MspⅠ ,HinfⅠ +MspⅠ对EV菌 16S - 2 3SrRNA基因间区扩增产物进行了双酶切分析 ,作出了TaqⅠ ,MspⅠ ,HinfⅠ在EV菌该间区的酶切位点图。结论 :鼠疫耶尔森氏菌 16S - 2 3SrRNA基因间区相当保守 ;
In this study the spacer region between the genes coding for 16S-23S rRNAs and a portion of the gene coding for 23S rRNA (spacer+23S) were selected for analysis. 103 Y.pestis strains that isolated from 9 different gorups of natural foci and belong to 21 ecotypes were studied. It was found that all of the tested strains showed two identical fragments of 1 146 bp and 1 090 bp. The amplified products were digested with two restriction endonucleases Taq Ⅰ and Msp Ⅰ,the result showed that the restriction patterns were identical. The physical map of 16S-23S rRNA gene spacer region of EV strains with the restriction endonucleases Taq Ⅰ, Msp Ⅰ and Hinf Ⅰ were constructed, it might be useful for further study of this region.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2000年第3期180-183,共4页
Chinese Journal of Vector Biology and Control
