摘要
目的 :观察肾综合征出血热 (HFRS)乳鼠脑纯化Ⅰ型灭活疫苗在浙江省HFRS高发疫区大面积人群接种后的安全性 ,考核血清学及近期、中期流行病学效果。方法 :采用间接免疫荧光法 (IFAT)检测特异性IgG抗体 ,用微量细胞病变中和试验 (MCPENT)检测中和抗体滴度。结果 :共接种 10 4 6 0人 ,全程接种者占 97.30 % ,对照 16 159人。全程接种后两周进行血清学观察 ,荧光抗体阳转率为 10 0 .0 0 % (6 7/ 6 7) ,中和抗体阳转率为 4 4 .4 4 % ,几何平均滴度分别为 72 .12和 4 .6 2。1年后加强免前、免后两周和免后 1年 ,荧光抗体阳性率分别为 2 8.57%、83.33%、75.0 0 % ;中和抗体阳性率分别为 14.80 %、55.56 %、35.0 0 %。疫苗近期 (基础免疫后 1年 )和中期 (基础免疫后 2年 )流行病学防病效果良好 ,人群保护率达 10 0 %。目前未发现接种人群中有免疫 (感染 )增强问题。结论 :HFRS疫苗近期和中期流行病学防病效果明显 。
Objective:Observe the safety, serological efficacy, short term and long term epidemiological efficacy character of Type Ⅰ inactivated HFRS vaccine in population in the focus of HFRS with high incidence of HFRS in Zhejiang province. Methods used in antibody test: Immunofluorecent antibody assay was used in testing specific IgG antibody and Mcro CPE method was used in testing the titer of neutralizing antibody. Result: 10 460 people were vaccinated, 97.30 % of them completed their vaccination of full doses. 16 159 non vaccinated people living in the same areas as the vaccinees were used as the control group. Two weeks after the injection of the third dose, the sero conversion rate by immunofluorecent antibody test (IgG) and neutralization test were 100.00 %(67/67) and 44.44 %, the GMTs of them were 72.12 and 4.62 respectively. Before reinforce,two weeks and 1 year after reinforce. The rates of seroconversion of immunofluorescent antibody by IFA method were 28.57 %, 83.33 % and 75.00 %. The rates of seroconversion of neutralizing antibody by the Mcro CPE method were 14.80 %, 55.56 % and 35.00 %. According to the short term (1 year after primary immunization) and middle term (2 years after primary immunization) observation, the vaccine was effective, and his protection rate in population was 100.00 %. Till now, we have not find any antibody dependent immunization enhancement phenomenon among the inoculated population. Conclusion:HFRS vaccine was epidemiologically effective in the short term and middle term.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2000年第3期169-172,共4页
Chinese Journal of Vector Biology and Control
基金
国家"九五"攻关课题!(96 - 90 6 - 0 3- 13)
关键词
肾综合征出血热
灭活疫苗
免疫增强
免疫保护
Hemorrhagic fever with renal syndrome(HFRS)
Inactivated vaccine
Protection effect
Antibody dependent immunization enhancement
Immunological strategy
