摘要
用PCR技术对华南地区分离的17株不同血清型副猪嗜血杆菌菌株ompP2基因进行克隆鉴定,并以CLUSTALS1和PHYLIP-3.68软件将ompP2基因序列进行比对和遗传进化分析。17株副猪嗜血杆菌菌株中均能扩出ompP2基因,克隆测序结果发现ompP2基因大小有所不同,与参考序列ABKM01000007的同源性在92%～99%之间。序列比较结果显示,ompP2基因与GenBank公布的副猪嗜血杆菌全基因组测序中的ompP2基因序列具有较高的同源性,不同菌株ompP2基因序列大小存在差异,为进一步验证ompP2蛋白的功能及相关研究奠定了基础。
We clarified the genetic structure and phylogenetic relationship of the ompP2 gene in Haemophilus parasuis,a gene encoding outer membrane protein.PCR was used to determine the ompP2 gene of 15 H.parasuis local isolates and 2 reference strains.Then we sequenced the ompP2 gene of H.parasuis.All ompP2 gene sequence from H.parasuis,were analyzed for phylogenetic relationship.An approximately 1.1 kb fragment was obtained from the genomic DNA of 17 H.parasuis strains.Sequence analysis showed that the size of the ompP2 gene was difference,the nucleotide sequence similarity was 92%-99% between 17 H.parasuis isolates and ABKM01000007.No obvious difference was found in the ompP2 gene sequence of 15 local isolates and 2 reference strains.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第1期29-31,93,共4页
Chinese Journal of Veterinary Science
基金
广东省农业攻关项目(2011B02036002)
广州市科技计划资助项目(2010Y1-C311)
