摘要
选用3头自然感染仔猪和1头阴性对照猪,剖杀后采集心脏、肝脏、脾脏、肺脏、肾脏、胰脏、脑、空肠、扁桃体、胸腺、淋巴结、膀胱、性腺等组织,用荧光定量PCR方法检测病毒在组织中的分布及病毒载量。结果显示,类猪圆环病毒因子P1分布在仔猪的心脏、肝脏、肺脏、胰脏、脑和膀胱等组织,病毒载量以胰脏、脑和膀胱为最高,可达105拷贝/g以上;对照猪脏器中没有检测到病毒核酸。该研究为P1的诊断及致病机制奠定了基础。
The objectives of this study were to investigate the distribution of porcine circovirus-like agent P1 in the tissues of piglets.Three naturally infected pigs and one negative-control pig were selected and sacrificed.The presence and viral load of P1 in collected tissue samples（including mesenteric and superficial inguinal lymph nodes,thymus,tonsil,heart,lung,kidney,liver,spleen,brain,pancreas,bladder,jejunum and gonad） were determined by real-time quantitative PCR.The results indicate that P1 may be distributed in heart,lung,liver,brain,pancreas and bladder.Across these tissues of naturally infected pigs,the brain,pancreas and bladder had the highest viral burdens with 105copies/g.Results from this study lay the foundation for diagnosis and pathogenesis studies of P1.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第1期1-3,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30972184)
江苏省自然科学基金资助项目(BK2008351)
