摘要
目的 研究大鼠脊髓损伤后胚胎脊髓 (FSC)移植是否能够影响胶质纤维酸性蛋白(GFAP)的表达和伤后大鼠后肢功能的恢复情况。 方法 将 6 0只大鼠分为脊髓半切洞损伤 +胚胎脊髓移植组 (A组 )和单纯脊髓半切洞损伤组 (B组 )。伤后 1,3 ,7,14,2 8d ,应用行为学和电生理检查观察大鼠功能恢复情况 ,应用原位杂交和免疫细胞化学方法观察GFAP的表达 ,并采用计算机图像分析技术 ,进行定量分析。 结果 大鼠脊髓损伤后GFAP的表达A组明显高于B组 ,A组损伤脊髓高表达GFAP持续至伤后 2 8d ,B组仅持续至伤后 14d。增加的GFAP阳性细胞数目与神经功能的改善相一致。 结论 胚胎脊髓移植能诱导大鼠损伤脊髓高表达GFAP免疫反应和GFAPmRNA蛋白 。
Objective To investigate whether fetal spinal cord (FSC) transplantation effected the expression of the glia fibrillary acidic protein (GFAP) mRNA and immunoreaction of GFAP as well as functional recovery after spinal cord injury in rats. Methods Experimental rats were divided into 2 groups, spinal cord hemisection injury combination with transplantation of FSC to injured site group (Group A), spinal cord injury and recept of gelfoam pledgets group (Group B). Rats were killed at 1, 3, 7, 14, 28 days. Then, open field and inclined plane tests, somatosensory evoked potentials(SEP) and motor evoked potentials(MEP) were examined. An in situ hybridization and immunocytochemistry was used to examine the spinal cord lesion and neighbor areas. The positive cells were quantitatively analysed by a computer image analysis system. Results In Group A, the number of cell expression protein GFAP mRNA and GFAP immunoreaction was more than that in Group B. By 28 days following injury, positive astrocytes cell of Group A returned to normal level but, the positive cells increased by 14 days postinjury in Group B. These increases in GFAP positive cells were paralleled by a significant improvement in neurological function recovery. Conclusions FSC transplantation is sufficient to reduce multiple aspects of gliosis and can promote neurological function recovery of adult rats after spinal cord injury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2000年第7期419-422,共4页
Chinese Journal of Trauma
基金
全军医学重点实验室资助项目!(97015)
关键词
脊髓损伤
胚胎
脊髓移植
胶质纤维酸性蛋白
Spinal cord injury
Spinal cord, fetus, transplantation
In situ hybridization
Immunohistochemistry
Glia fibrillary acidic protein
