摘要
目的研究不同浓度安氟醚（Ｅｎｆ）对常温或低温下心肌能量代谢的影响。方法２４只家兔的离体心脏， Ｌａｎｇｅｎｄｏｒｆｆ装置平衡灌注 １５ｍｉｎ后，随机分为 ４组（ ｎ＝ ６）， Ａ组：常温 １． １％ Ｅｎｆ的Ｋ－Ｈ液灌注； Ｂ组：常温 ２． ２％ Ｅｎｆ的 Ｋ－Ｈ液灌注； Ｃ组：低温 １． １％ Ｅｎｆ的 Ｋ－Ｈ液灌注； Ｄ组：低温２． ２％ Ｅｎｆ的 Ｋ－Ｈ液灌注。常温组温度为 ３７℃，低温组温度为 ３０℃。灌注 ３０ｍｉｎ后，取心失部心肌组织测定腺苷酸含量、ＡＴＰ酶活性。结果常温或低温下， ２． ２％ Ｅｎｆ组 ＡＴＰ含量明显高于 １． １％ Ｅｎｆ组，Ｎａ＋－Ｋ＋ＡＴＰａｓｅ活性明显低于１．１％ Ｅｎｆ组（Ｐ＜０．０５或０．０１）；而低温下，２．２％ Ｅｎｆ组ＡＤＰ含量及ＥＣ明显高于１．１％ Ｅｎｆ组，Ｃａ２＋ＡＴＰａｓｅ活性明显低于１．１％ Ｅｎｆ组（Ｐ＜０．０５或０．０１）。结论 Ｅｎｆ通过抑制心肌细胞膜ＡＴＰａｓｅ活性导致心脏电生理受到抑制，低温可能加重安氟醚的心肌抑制作用。
Objective To investigate the effects of enflurane (Enf)at normothermia or hypothermia on myocardial energy metabolism. Methods Twenty-four isolated rabbit hearts, perfused with modified Krebs-Hensleit buffer using Langendorff's device in 15 min, were randomly assigned to be perfused with 1. 1 % Enf at normothermia (37℃ ), 2. 2 % Enf at normothermia (37℃ ), 1. 1 % Enf at hypothermia (30℃ ), or 2. 2 % Enf at hypothermia (30 ℃ ), respectively. The myocardial adenine nucleotides contents, activities of ATPases were measured after 30min perfusion. Results In group 2.2% Enf, ATP content was significantly higher and the activity of Na+ -K+ ATPase was significantly lower than those in group 1. 1 % Enf at normothermia or hypothermia (P < 0. 05 or 0. 01 ); but ADP content and EC in group 2. 2 % enflurane were obviously higher and activity of Ca2+ ATPase was lower than those in group 1. 1 % Enf at hypothermia (P < 0. 05 or 0. 01 ). Conclusions The Enf-mediated depressions of cardiac eletrophysiology and contractility may result from inhibiting the activity of cellular membrane ATPase to reduce myocardial energy metabolism, which can be aggravated by hypothermia.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第7期429-431,共3页
Chinese Journal of Anesthesiology
关键词
低温
能量代谢
心肌
安氟醚
Hypothermia
Enflurane
Energy metabolism
Myocardiu
