摘要
目的:观察不同浓度的磁性纳米颗粒对肝癌HepG2细胞生长的影响。方法:化学共沉淀法制备磁流体,以浓度0、4和8mg/mL的磁微球分别作用于HepG2细胞,48h后流式细胞仪检测细胞凋亡情况。以不同浓度的磁微球作用于HepG2细胞不同时间,MTT检测其增殖率的变化。电镜观察磁微球作用于肝癌HepG2细胞后的形态学变化。结果:制备的磁流体为黑色胶体混悬液,透射电镜观察发现,颗粒以椭圆形和球形居多,颗粒大小多为70nm左右。4mg/mL磁流体作用于HepG2细胞48h后的凋亡率为(32.73±4.80)%,8mg/mL磁流体作用于HepG2细胞48h后的凋亡率为(53.87±5.35)%,与HepG2细胞自身凋亡率的(21.15±1.06)%相比,差异均有统计学意义,P值均<0.05。磁微球浓度为4mg/mL分别作用HepG2细胞24和48h的细胞抑制率分别为17.97%和20.22%、8mg/mL则为35.93%和35.27%,与磁微球浓度为0mg/mL作用相同时间的抑制率比较差异均有统计学意义,t值分别为2.237,2.483,2.985和3.173,P值均<0.05。结论:磁微球可对抑制肝癌HepG2细胞增殖,但不成剂量时间依赖关系。
OBJECTIVE： To detected the growth of hepato-cellular carcinoma HepG2 cells after treated by different concentrations of magnetic nanoparticles, and observe them with electron microscopy. METHODS： Preparated magnetic fluid with chemical coprecipitation. The concentration of 0 mg/mL, 4 mg/mL and 8 mg/mL of the magnetic mierospheres, respectively role in in HepG2 cells. After 48 hours,cells apoptosis was detected by using flow cytometry different concen- trations of magnetic microspheres effected on HepG2 cells at different time,and detected the change of proliferation rate with MTT. The different cell morphology was observed after magnetic microspheres had effected on HepG2 liver cancer cells with electron microscope. RESULTS： The magnetic nanoparticles were black colloidal suspension. The majority of magnetic nanoparticles were elliptical and spherical. The particles size mostly were about 70 nm. The apoptosis rates of HepG2 ceils treated by 4 mg/mL magnetic microspheres for 48 h were （32.73-t-4.80）~, and 8 mg/mL were （53.87 ＋ 5.35 ）%. There were statistically significant compared with H epG2 cells apoptosis rates （21.15±1.06）% （all P〈0.05）. The apoptosis rates of HepG2 cells treated by 4 mg/mL magnetic microspheres for 24 and 48 h were 17. 97%and 20.22%, 8 mg/mL were 35.93% and 35.27%, there were statistically significant compared with the apoptosis rates of 0 mg/mL magnetic microspheres treated by the same time（t values were 2. 237,2. 483,2. 985 and 3. 173, and all P values 0.05）. CONCLUSION： Magnetic microspheres can inhibit the proliferation of hepatoma HepG2 cells,but not a dose time dependent.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第24期1860-1862,1874,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
黑龙江省科技攻关计划重点项目基金(GB05C402-07)
黑龙江省教育厅科技项目基金(11521183)
