摘要
目的探讨噻托溴铵对瘦素促大鼠气道平滑肌细胞(ASMCs)增殖的作用。方法体外培养大鼠ASMCs,瘦素(100μg/L)干预后,加入不同浓度噻托溴铵(μg/L)(15、30、60和120),CCK-8法测定ASMCs增殖,荧光实时定量RT-PCR及免疫印迹(Western blot)分别检测瘦素受体mRNA及蛋白含量水平表达。结果与对照组比较,瘦素可明显促进大鼠ASMCs增殖(P<0.05),加入噻托溴铵则抑制瘦素的促增殖作用(P<0.05),并与浓度相关(P<0.01)。瘦素作用后,其受体mRNA表达增加,蛋白含量由对照组的0.327±0.011升至0.541±0.016(P<0.05);噻托溴铵干预后,瘦素受体mRNA表达减少,蛋白含量分别回降至(0.514±0.017、0.468±0.025、0.331±0.023、0.313±0.017)(P<0.05);并与浓度相关(P<0.01)。结论噻托溴铵可抑制瘦素的促ASMCs增殖作用,其机制可能与下调大鼠ASMCs表面的瘦素受体表达有关。
Objective To explore the effects of tiotropium on the proliferation of rats＇ airway smooth muscle cells by leptin stimulation.Methods ASMCs were collected from rat airway tissue and cultured in vitro.Then ASMCs were treated with leptin（the final concentration 100 μg/L） and tiotropium at different concerntrations（μg/L）（15,30,60 and 120） and cell proliferation was measured by CCK-8 assay.Realtime RT-PCR and Western blot were used to verify the expressions of leptin receptors on ASMCs.Results Leptin promoted the proliferation of ASMCs compared with control group（P0.05）.After treatment with tiotropium,leptin＇s promotion of the proliferation of ASMCs was inhibited in a dose-dependent manner（P0.01）.After treatment with leptin,the expressions of leptin receptor mRNA were increased and the proteins were rose from 0.327±0.011 to 0.541±0.016.The expression of leptin receptors was positively related to tiotropium concentrations（P0.01）.Conclusions Tiotropium can inhibit the proliferation of rats＇ airway smooth muscle cells stimulated by leptin,and its mechanism may be correlated to decreased expression of leptin receptors on rats＇ ASMCs.
出处
《基础医学与临床》
CSCD
北大核心
2013年第1期98-101,共4页
Basic and Clinical Medicine
关键词
噻托溴铵
瘦素
瘦素受体
气道平滑肌
增殖
tiotropium
leptin
leptin receptor
airway smooth muscle cells
proliferation
