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假互补肽核酸及其分子生物学效应

The Molecular Biological Effects of Pseudocomplementary Peptide Nucleic Acids
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摘要 假互补肽核酸(pseudocomplementary peptide nucleic acids,pcPNAs)是肽核酸(peptide nucleicacids,PNA)的一种衍生物,通过碱基修饰可以使pcPNAs同时与双链DNA两条链中相应的靶序列结合;而在pcPNAs识别靶序列并结合时,pcPNAs自身两条链间由于空间位阻作用,不会自身互补结合.pcPNAs与DNA、RNA甚至肽核酸相比具有独特的杂交特性,因此具有非常广泛的分子生物学效应.本文就pcPNAs寡聚体的结构、与核酸杂交的特点及杂交模式,分子生物学效应以及应用等方面进行介绍. Pseudocomplementary peptide nucleic acids（pcPNAs） are the modified derivative of peptide nucleic acids（PNA）, the pcPNAs are therefore synthesized to contain 2,6-diaminopurine and 6-thiouracil instead of adenine and thymine. Due to steric hindrance, pcPNAs are unable to form pcPNA-pcPNA duplexes but can bind to complementary DNA sequences by Watson-Crick pairing via double duplex-invasion complex formation. pcPNAs have uniqe hybridization characteristics compared with DNA / RNA, and peptide nucleic acid ,so have a very wide range of molecular biological effects. This article overviewed pcPNAs oligomeric structure, hybrid characteristics , hybrid models, molecular biological effects and applications.
作者 季明辉 欧青叶 顾大勇
机构地区 深圳出入境检验检疫局 珠海市结核病防治所
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2013年第1期19-24,共6页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金课题(No.30972827) 国家质量监督检验检疫总局科技项目(No.2009IK225)~~
关键词 假互补肽核酸 肽核酸 双链DNA pcPNAs PNA double-stranded DNA
分类号 Q617 [生物学—生物物理学] Q71 [生物学—分子生物学]
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参考文献31

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中国生物化学与分子生物学报
2013年 第1期

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