摘要
目的观察附件扭转(AT)后再灌注对兔卵巢丙二醛(MDA)、谷胱甘肽-过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的影响。方法雌性成年日本大耳白兔40只,随机分为实验组(n=32)和对照组(n=8)。实验组兔将左侧附件按顺时针方向扭转3周后固定于左侧腹壁,24h后解除扭转,再分成4组,每组8只,分别于24、48、72、96h后取双侧卵巢。对照组行假手术,96h后取双侧卵巢。取左侧卵巢检测GSH-Px、CAT活性及MDA含量,以切除的右侧卵巢作为内对照。结果附件扭转解除后,GSH-Px活力于再灌注24h至72h明显下降(P<0.01),96h后与对照组相比差异无统计学意义(P>0.05);CAT活力在附件扭转解除后24h及48h明显下降(P<0.01),72h后开始升高,与对照组比较差异无统计学意义(P>0.05),而96h后明显高于对照组(P<0.05);附件扭转解除后24h及48h,实验组左侧卵巢MDA含量明显上升(P<0.01),72h后开始下降,但仍明显高于对照组(P<0.01),至96h时与对照组比较差异无统计学意义(P>0.05)。结论附件扭转后解除扭转,GSH-Px、CAT和MDA发生变化,影响家兔卵巢的氧化应激性损伤程度,随着解除扭转时间的延长,卵巢损伤逐渐减轻,这与卵巢局部产生的GSH-Px、CAT逐渐恢复或升高,同时MDA逐渐清除有关。
Objective To observe the influence of reperfusion after adnexal torsion（AT） on malondialdehyde（MDA）,glutathione peroxidase catalase（GSH-Px） and catalase（CAT） contents of ovarian tissue in rabbits.Methods Forty female Japanese long-eared white rabbits were randomly divided into study group（n=32） and control group（n=8）.The left adnexa of rabbits in the study group was clockwise twisted three laps,and then fixed on the left abdominal walls.Adnexal detorsion was then done 24 hours after adnexal torsion in the study group,and then the rabbits were divided into 4 groups（8 each）.Both ovaries of each group were removed 24h,48h,72h and 96h,respectively,after reperfusion.The rabbits in the control group received sham-operation and both ovaries were removed 96h later.The removed left ovaries were used for biochemical detection of GSH-Px,CAT and MDA.All the right ovaries were used as experimental internal-control.Results The activity of GSH-Px declined significantly 24h to 72h after adnexal detorsion（P〈0.01）,and no significant difference was found when compared with that of control group 96h later（P〈0.05）.The activity of CAT declined significantly 24h and 48h after adnexal detorsion（P〈0.01）,then it began to increase after 72h,and it became significantly higher in 96h group as compared with that in control group（P〈0.05）.MDA content in study groups increased obviously 24h and 48h after adnexal detorsion（P〈0.01）,and it declined 72h later albeit still significantly higher than that of control group（P〈0.01）,and then it continuously declined to reach the level of control group 96h later（P〈0.05）.Conclusions Detorsion after adnexal torsion can affect the degree of oxidative stress injury in rabbits＇ ovaries as shown by the changes in the activities of GSH-Px,CAT and MDA.With the elongation of detorsion time,ovarian injury will be gradually alleviated.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2013年第1期23-25,共3页
Medical Journal of Chinese People's Liberation Army
基金
辽宁省自然科学基金(201102233)~~
