摘要
目的和方法 :用Fura 2 /AM .荧光显示测定细胞内游离Ca2 + 浓度 ([Ca2 + ]i)的方法 ,我们研究了牛磺酸 (Tau)对血管紧张素Ⅱ (AngⅡ )引起的培养心肌细胞 [Ca2 + ]i变化的影响。结果 :在有Ca2 + 和无Ca2 + 的缓冲液中 ,AngⅡ (1 ,1 0 ,1 0 0 ,1 0 0 0nmol/L)能浓度依赖性地引起 [Ca2 + ]i升高。在含Ca2 + 的缓冲液中 ,Tau(1 0 ,2 0mol/L)可依浓度地抑制AngⅡ (1 0 0nmol/L)引起的 [Ca2 + ]i升高 ;但在无Ca2 + 的缓冲液中 ,牛磺酸无此作用。用ryanodine(Rya ,1 μmol/L)预先耗竭细胞内贮存的Ca2 + 后 ,AngⅡ (1 0 0nmol/L)仍能引起 [Ca2 + ]i进一步升高 :AngⅡ的这一作用能被Tau(2 0mmol/L)显著抑制。结论 :在培养的乳鼠心肌细胞 ,Tau能够通过抑制AngⅡ引起的Ca2 + 内流而拮抗AngⅡ升高 [Ca2 + ]i的作用。
Aim and Methods: The effects of taurine (Tau) on the Ca 2+ movement induced angiotensin Ⅱ were observed in cultured myocardial cells of neonatal rats with the fura 2 probe. Results: Ang Ⅱ (1,10,100,1 000 nmol/L) increased [Ca 2+ ]i dosedependently in both Ca 2+ containing medium and Ca 2+ free medium. The rising [Ca 2+ ]i evoked by AngⅡ (100 nmol/L) were decreased by Tau(10,20 mmol/L)in a concentration dependent manner in Ca 2+ medium but not in Ca 2+ free medium. After depletion of intracellular Ca 2+ pools with ryanodine (1 μmol/L), AngⅡ caused another increase in [Ca 2+ ]i. This Ang Ⅱ induced increase in [Ca 2+ ]i was markedly inhibited by Tau (20 mmol/L). Conclusion: These results indicate that the mechanism of antagonistic effects of Tau on Ang Ⅱ-induced Ca 2+ movement is related to Tau inhibitory action on the Ca 2+ influx evoked by Ang Ⅱ.
出处
《中国应用生理学杂志》
CSCD
2000年第1期26-29,共4页
Chinese Journal of Applied Physiology
