摘要
采用多孔聚酯泡沫固定里氏木霉 ,在鼓泡柱固定化反应器中同时产酶降解壳聚糖。结果表明通过控制降解时间可以得到不同平均聚合度的降解产物。在 2 8℃ ,pH4 8,通气量 3vvm条件下 ,利用固定化反应器 ,在 30d内连续进行 10批同时产酶降解试验 ,结果发现壳聚糖酶活力和壳聚糖降解率能保持稳定。每批产生的壳聚糖酶活力平均达到 0 15u/mL以上 ,壳聚糖平均降解率为 73%。
The cells of Trichoderma reesei were immobilizxed on a roll of porous polyurethane foam sheet and packed in a bubble column bioreactor for simultaneous production of chitosanase and degradation of chitosan in situ . The average degree of polymerization could be regulated by reaction time.Under the repeated\|batch process with 2% soluble chitosan at pH4\^8,28℃,the activity of chitosanase for each batch was above 0\^15 IU/mL,the average yield of reducing sugar as D \|glucosamine reached 73%.The novel immobilized bioreactor system run stably and effectively in the successive 10 batches lasting 30 days without notable change in the activity and productivity.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第3期368-372,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金资助项目! ( 2 98763 6)
关键词
里氏木霉
固定化反应器
产酶降解耦合
壳聚糖
Trichoderma reesei ,convoluted fibrous bed bioreactor,chitosanase production and hydrolysis of chitosan
