摘要
以 3个大豆 (GlycinemaxL .)品种 (“浙春 3号”、“浙春 2号”和“3811”)为材料 ,设置不同的钼、硼水平 ,研究了 3个生育期 (五叶期、初花期和盛荚期 )大豆叶片膜脂过氧化及体内保护系统的变化。结果表明 :在低钼、低硼胁迫下 ,大豆叶片的质膜透性 (MP)、丙二醛 (MDA)和脯氨酸 (Pro)的含量、多酚氧化酶 (PPO)和抗坏血酸氧化酶 (AO)的活性增加 ,抗坏血酸 (AsA)的含量及超氧化物歧化酶 (SOD)、过氧化物酶 (POD)、抗坏血酸过氧化物酶 (AP)、过氧化氢酶 (CAT)的活性下降 ;施钼或施硼都有利于大豆抗膜脂过氧化胁迫 ;大豆体内保护系统中的酶类 (SOD、POD、CAT和AP)和非酶类化合物 (AsA和Pro)密切相关 ,协同抗氧化 ;在抵御膜脂过氧化的程度上 ,钼和硼的作用存在一定的差异 ,且钼和硼呈现相互促进的作用 ;3个大豆品种对钼、硼的反应存在基因型差异 ,与抗氧化系统的整体活力(酶类和非酶类 )相关。
The study deals with the changes of membrane peroxidation and endogenous protective system with different supplementation of molybdenum (Mo) and/or boron (B) concentration in soybean (Glycine max L.) leaves at three developmental stages (5_trifoliate stage, initiation of flowering, and peak podsetting stage) in three pot_grown soybean varieties (“Zhechun No.3”, “Zhechun No.2”, “3811”). The control plants under low Mo and low B exhibited an increasing of membrane permeability (MP), malondialdehyde (MDA) and proline (Pro) contents, polyphenol oxidase (PPO) and ascorbate oxidase (AO) activities and a decrease of ascorbate (AsA) contents, superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (AP) and catalase (CAT) activities. Application of Mo or B alone raised the ability of anti_oxidant of soybean leaves. The results indicated that the anti_oxidant enzymes (including SOD, POD, CAT and AP) related closely to anti_oxidant compounds (including AsA and Pro). There was some difference between the effects of Mo and B on the anti_oxidant, and a synergetic effect was observed between Mo and B. Some genetic variation in the responses to Mo and B was found among the three soybean varieties which was related to the activities of the total anti_ oxidant systems.
基金
澳大利亚国际农业中心基金 !(PN912 0 )
浙江省分析测试基金!( 990 0 2 5 )&&
关键词
大豆
钼
硼
膜脂过氧化
体内保护系统
抗胁迫
soybean
molybdenum
boron
membrane lipid peroxidation
endogenous protective system
