摘要
目的探讨嘌呤受体P2X配体门控性离子通道7（P2X7R）在初发系统性红斑狼疮（SLE）患者外周血淋巴细胞上的表达及其与部分炎症细胞因子的相关性。方法选择29例初发SLE患者及28名健康对照，分离外周血单个核细胞（PBMC），采用流式细胞术检测淋巴细胞、CD4＋淋巴细胞、CD19＋淋巴细胞上P2X7R的表达水平，酶联免疫吸附试验（EusA）检测P2X7R相关的细胞因子白细胞介素（IL）-1β、IL-6及肿瘤坏死因子（TNF）-α水平。采用t检验、Wilcoxon秩和检验和Spearman相关分析进行统计学分析。结果①SLE患者外周血CD4-淋巴细胞、CD19＋淋巴细胞表面P2X7R的表达明显高于健康对照组[CD4＋淋巴细胞：2．21（3．55）和0．89（1．15），Z=-1．527，P=0．015；CD19＋淋巴细胞：11．53（20．01）和6．66（6．27），Z=-2．091，P=-0．037]；②SLE患者血清中3种细胞因子水平均明显高于健康对照组，差异有统计学意义；SLE患者外周血淋巴细胞中P2X7R表达水平与IL-6呈正相关（r=0．449，P=0．015）；③SLE患者中，关节炎组淋巴细胞上P2X7R表达显著高于非关节炎组[3．84（11．53）与0．90（1．81），Z=-2．772，P=0．006]；P2X7R在淋巴细胞及CD19＋淋巴细胞中的表达均与SLE疾病活动指数（SLEDAI）评分呈正相关；淋巴细胞上的P2X7R表达与抗B：糖蛋白I抗体呈正相关（r=0．575，P=0．008）。结论P2X7R可能通过介导炎症因子的释放参与SLE发病；可能与SLE患者的关节炎、狼疮肾炎及神经精神狼疮相关。
Objective To analyze the expression of purinergic receptor P2X ligand-gated ion channel 7 （P2X7R） on different cells and peripheral blood mononuclear cell （PBMC） and to investigate its correlation with inflammatory cytokines in patients with SLE. Methods Flow cytometry was used to detect surface expression of P2X7R on lymphocytes, CD4＋ cells, and CD19＋ cell in 29 SLE patients and 28 healthy human controls to compare the difference between the SLE patients and the controls in P2X7R expression. Enzyme linked immunosorbent assay （ELISA） was performed to detect P2X7R-related serum eytokines interleukin （IL）-l^3, IL-6, tumor necrosis factor （TNF）-ot level. T test, Wilcoxon rank sum test, Spearman＇s correlation analysis were used for statitieal analysis. Results （1） SLE patients had significantly higher expression of P2X7R on CD4＋, CD8＋ lymphocytes compared to controls [ CD4＋ cells： 2.21 （3.55） vs 0.89 （1.15）, Z=- 1.527, P=-0.015; CD19＋ cells： 11.53（20.01） vs 6.66 （6.27）, Z=-2.091, P=0.037]; （2） The levels of three cytokines in patients with SLE were significantly higher than those in control. The positive relationship between P2X7R expression in lymphocytes with the serum IL-6 level was found in SLE patients （r=0.449, P=0.015）; （3） Patients with arthritis showed significantly higher expression of P2X7R on lym-phocytes compared to patients without arthritis （Z=-2.772, P=0.006）. The expression of P2X7R on lymphocytes and CD19＋ cell was significantly positively correlated with the SLEDAI score. Positive correlation with anti-β2GP I in lymphocytes was also found. Conclusion P2X7R may mediate the release of inflammatory cytokines involved in the pathogenesis of SLE, and may participate the development of arthritis, lupus nephritis and NPSLE in SLE patients.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2013年第1期46-48,共3页
Chinese Journal of Rheumatology
基金
安徽省自然科学基金（1208085MH141）
安徽省卫生厅青年科研项目（098121）
