摘要
诱导性冷失活是 V型 ATPase的一种共同特性。用荧光光谱技术以水稻液泡膜 H+ -ATPase为研究对象 ,深入探讨了水稻幼苗冷失活现象的机制。研究表明 ,能引起较大程度冷失活现象的物质 ,往往先对膜脂有序性产生影响 ,通过对多种阳离子、阴离子、离子螯合剂导致冷失活能力和导致膜脂有序性变化能力间的比较 ,都能说明这一点。可以认为膜脂状态变化是冷失活机制发生的一个重要方面 ,也是植物早期寒害产生的原因之一。
Induced cold inactivation is a general characteristic of V type H + ATPase.In this paper,fluorescence spectrum technique has been used to thoroughly study the mechanism of cold inactivation of H + ATPase on rice tonoplast.It shows that the chemicals that can cause the cold inactivation considerably usually cause the destruction of the order of tonoplast lipid first.The capability of inducing cold inactivation and changing the order of membrane lipid in cations,anions and chelation agents has been testified and compared.The result proved the conclusion above.We consider that the changing of membrane lipid in tonoplast plays an important role in cold inactivation and is one of the reasons of early cold pet of plants.
出处
《武汉植物学研究》
CSCD
2000年第2期123-126,共4页
Journal of Wuhan Botanical Research
基金
国家自然科学基金资助项目!( 3 9570 4 3 4 )
