摘要
[目的 ]检测血吸虫病患者的循环抗原。 [方法 ]用多抗与单抗夹心 EL ISA法检测日本血吸虫病患者血清循环抗原。 [结果 ]用于检测 15 0例血吸虫病患者的循环抗原 ,其敏感性平均为 84.7% (72 .0 %～ 91.0 % ) ,40例正常人未出现假阳性反应 ,74例其他寄生虫感染者中 ,有 2例并殖吸虫病患者阳性 ,其余均未出现交叉反应。 [结论 ]多抗与单抗夹心 EL ISA法是一种较为理想的循环抗原检测方法。
Objective] To detect circulating antigen in the patients with schistosomiasis. [Methods] Polyclonal antibody(PcAb) monoclonal antibody(McAb) sandwich ELISA. [Results] Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84 7%(72 0%～91 0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections. [Conclusion] PcAb McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.
出处
《中国寄生虫学与寄生虫病杂志》
CSCD
北大核心
2000年第2期92-93,共2页
Chinese Journal of Parasitology and Parasitic Diseases
基金
欧共体资助!(合同号 :IC 18C T97-0 2 41)
‘九五’科技攻关课题
