期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

缺血／再灌注不同时间点大鼠海马基质细胞诱导因子1α和趋化因子受体4的表达变化

Expressional changes of stromal cell-derived factor 1α and C-X-C chemokine receptor type 4 protein in the hippocampus of ischemia/reperfusion rat
暂未订购
导出
摘要 目的：研究脑缺血损伤后大鼠海马基质细胞诱导因子1α（SDF-1α）和CXCR4蛋白的表达变化情况。方法：运用免疫组织化学和免疫印迹检测SDF-1α和CXCR4的表达变化。结果：免疫组织化学显色显示，大鼠海马CA区和DG区均可见SDF-1α和CXCR4表达阳性的细胞，其胞质呈棕黄色，DG区主要表达于齿状回颗粒细胞下层。表达SDF-1α和CXCR4的阳性细胞数均随时间推移而增多。免疫印迹检测进一步表明，脑缺血／再灌后1、3、7d和1个月均可见SDF-1α及CXCR4蛋白表达条带，且SDF-1α的表达随时间而增高，与1d比较，复灌7d、1个月时表达差异有统计学意义；CXCR4蛋白表达也随复灌时间延长而增加，但各组之间的表达无差异。结论：缺血／再灌脑损伤后SDF-1α蛋白表达具有时间差异性，提示SDF-1α在缺血性脑损伤过程中可生成、释放，其与缺血性脑损伤之间存在一定的关系。 Objective： To investigate expression changes of stromal cell-derived factor 1α （SDF-1α） and C X-C chemokine receptor type 4 （CXCR4） proteins in the hippocampus of the ischemia/reperfusion model of rats. Methods： Immunohis tochemistry and Western blotting analyses were used to detect SDF-1 α and CXCR4 expression in the hippocampus of is ehemia/reperfusion rats. Results： Immunohistochemistry showed that the SDF-1 α and CXCR4 were expressed in the CA and DG fields of the hippoeampus, in which cytoplasm was stained, and majority of the positive ceils was expressed in the subgranular zone. The positive cell number of SDF-1α and CXCR4 was increased with extending of time. Western blotting results showed that the protein of SDF-1α and CXCR4 was expressed in the hippocampus at different ischemic/ reperfusion time. The protein expressions of SDF-1α and CXCR4 were increased gradually with time and there was sta tistically significant compared with 1 day in the protein expression of SDF-1α. Conclusion： The SDF 1α expression is temporal different in the hippocampus of ischemic/reperfusion rats. This implies that the SDF-1α can be produced and re- leased during ischemic/reperfusion injury and may be associated with brain ischemic injury.
作者 王玉兰 符炜 李爱娜 徐红 刘志安 高殿帅
机构地区 徐州医学院 解剖和神经生物学教研室 徐州医学院 附属医院普外科
出处 《解剖学杂志》 CAS CSCD 北大核心 2012年第6期792-795,共4页 Chinese Journal of Anatomy
基金 国家自然科学基金（81171141） 徐州医学院院长专项人才基金（2010KJZ19）
关键词 基质细胞诱导因子1α 趋化因子受体4 缺血 再灌注 海马 stromal cell-derived factor 1α C-X-C chemokine receptor type 4 cerebral ischemia/reperfusion hippocampus
分类号 R654.1 [医药卫生—外科学]
  • 相关文献

参考文献11

  • 1Chang Y C, Shyu W C, Lin S Z, et al. Regenerative therapy for stroke [J].Cell Transplant, 2007,16(2) : 171-181.
  • 2Pulsinelli W A, Brierley J B. A new model of bilateral hemispher- ic ischemia in the unaesthetized rat [J]. Stroke, 1979,10(3):267 272.
  • 3Lowry O H, Rosebrough N J, Farr A L, et al. Protein measure- ment with the Folin phenol reagent [J].J Biol Chem, 1951,193 (1):265-275.
  • 4Gu Z, Jiang Q, Zhang G. c-Jun N-terminal kinase activation in hippocampal CA1 region was involved in ischemic injury [J]. Neuroreport, 2001,12(5) :897-900.
  • 5Basu S, Rav N T, Atkinson S J. et al. Protein phosphatase 2A plays an important role in stromal cell-derived factor-1/CXC che- mokine ligand 12-mediated migration and adhesion of CD34+cells [J]. J Immunol, 2007,179 (5) : 3075-3085.
  • 6Wang Y, Huang J, Li Y, et al. Roles of chemokine CXCL12 and its receptors in ischemic stroke [J]. Curr Drug Targets, 2012,13 (2) : 166-172.
  • 7Yu X, Chen D, Zhang Y, et al. Overexpression of CXCR4 in mesenchymal stem cells promotes migration, neuroprotection and angiogenesis in a rat model of stroke [J]. J Neurol Sci, 2012,316 (1-2) : 141-149.
  • 8Kucia M, Zhang Y P, Reca R,et al. Cells enriched in markers of neural tissue-committed stem ceils reside in the bone marrow and are mobilized into the peripheral blood following stroke [J]. Leu- kemia, 2006,20(1) : 18-28.
  • 9Robin A M, Zhang Z G, Wang L, et al. Stromal cell-derived fac- tor 1a mediates neural progenitor cell motility after focal cerebral isehemia [J]. J Cereb Blood Flow Metab, 2006,26(1): 125-134.
  • 10王玉兰,徐红,李爱娜.长期培养大鼠海马脑片CAl区基质细胞诱导因子和CXCR4蛋白的免疫活性变化[J].解剖学杂志,2011,34(6):778-780. 被引量：1

二级参考文献18

  • 1王玉兰,徐铁军,张凤真,彭裕文.海马脑片缺氧缺糖模型的制备方法[J].解剖学杂志,2005,28(5):604-606. 被引量：4
  • 2Rambani K, Vukasinovic J, Glezer A, et al. Culturing thick brain slices: an interstitial 3D microperfusion system for enhanced viability [J]. J Neurosci Methods, 2009, 180 (2) :243 - 54.
  • 3Cho S, Wood A, Bowlby M R. Brain slices as models for neurodegenerative disease and screening platforms to identify novel therapeutics[J]. Curr Neuropharmacol,2007,5 ( 1 ) : 19 - 33.
  • 4Lowry O H, Rosebrough N J, Farr A L, et al. Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951,193 (1) :265 -75.
  • 5Gu Z, Jiang Q, Zhang G. e-Jun N-terminal kinase activation in hippoeampal CA1 region was involved in isehemic injury [ J ]. Ncuroreport, 2001,12 ( 5 ) : 897 - 900.
  • 6Wang Y, Deng Y, Zhou G Q. SDF-lalpha/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells to- wards ischemic brain lesion in a rat model [J]. Brain Res,2008, 1195:104 - 12.
  • 7Rosenkranz K, Kumbruch S, Lebermann K, et al. The chemokine SDF-1/CXCR4 contributes to the " homing" of umbilical cord blood ceils to a hypoxic-ischemic lesion in rat brain[J]. J Neurosci Res,2010,88(6) :1223 -33.
  • 8Han Y, Zhang W. Simultaneous removal of NO (x) and SO2 in exhausted gas through landfill leachate [J]. Indian J Exp Biol, 2010,48(12) :1237 -42.
  • 9Rostene W, Kitabgi P, Parsadaniantz S M. Chemokines: a new class of neuromodulator[J]. Nat Rev Neurosci,2007,8 ( 11 ) :895 903.
  • 10Nassif M, Hoppe J, Santin K, et al. Beta-amyloid peptide toxici ty in organotypie hippocampal slice culture involves Akt/PKB, GSK-3beta and PTEN [J ]. Neurochem Int, 2007, 50 ( 1 ):229-235.
解剖学杂志
2012年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部