摘要
目的：观察糖尿病大鼠肾皮质内钾离子通道Kv1．3、Fas及FasL的表达变化。方法：SD雄性大鼠用链脲佐菌素复制糖尿病动物模型，分别于4周、12周后测体质量、尿蛋白、血糖、尿素氮及肌酐，H—E染色观察肾形态学变化，免疫组织化学观察Kv1．3通道蛋白、Fas和FasL表达变化及TUNEL法观察大鼠肾皮质细胞凋亡情况。结果：与正常对照组比较，糖尿病组大鼠尿蛋白、血糖、尿素氮及血肌酐增高。4周糖尿病组大鼠肾小球体积增大，12周组肾小球系膜基质增生和肾小球硬化，肾小管上皮细胞空泡样变。糖尿病组大鼠肾小管上皮细胞Kv1．3通道蛋白及Fas和FasL表达随病程延长显著增加。细胞凋亡检测结果显示，4周时凋亡细胞增多，多数在远曲肾小管，12周远曲肾小管及近曲肾小管均可见凋亡细胞。结论：Kv1．3通道蛋白和Fas及FasL表达随糖尿病病程延长而增强，Kv1．3通道蛋白表达可能参与Fas及FasL诱导的细胞凋亡，导致肾功能异常。
Objective： To investigate the change of expressions of Kv1. 3 and Fas, FasL in the renal cortex of diabetic rats. Methods： The Sprague-Dawley rat diabetic model was induced by injection of streptozotocin. After 4 and 12 weeks, the rats were sac- rifieed for assaying weight, urine protein, blood glucose, blood urine nitrogen and serum creatinine. The change of the renal morphol- ogy was observed by HE staining and immunohistochemical method was used to visualize the expression of Kv1. 3 and Fas/FasL and the renal apoptosis was determinated by TUNEL method. Results： Compared with the normal control group, urine protein, blood glucose, urine nitrogen and serum creatinine were significantly higher in diabetic group. In 4 weeks diabetic rats, glo- merulus increased in size. 12 weeks rats showed renal glomerulus mesangial matrix hyperplasia and glomerular sclerosis, vac- uolar degeneration in renal tubular epithelial cells. Kv1. 3 and Fas/FasL immunopositive cells showed a ascending tendency with the progression of the disease. Apoptosis tests showed that apoptotic cells increased in the distal tubular epithelial cells of 4 weeks group, and apoptotic cells in the distal tubular and proximal tubules were visible in 12 weeks group. Conclusion： The expression of Kvl. 3 and Fas/FasL in the kidney was gradually increasing with diabetes progressing. Kv1. 3 channel protein expression may be involved in Fas and FasL induction of apoptosis in the kidneys of diabetic rats, and lead to renal dysfunction.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第6期721-723,736,F0002,共5页
Chinese Journal of Anatomy
基金
安徽省教育厅高等学校省级优秀青年人才基金（2010SQRL174）
