摘要
目的:探讨S100A11和自噬基因Beclin1在胃癌组织中的表达及临床意义.方法:采用免疫组织化学超敏两步法(S-P法)分别检测50例胃癌组织、30例胃癌前病变(中、重度非典型增生)和20例慢性非萎缩性胃炎组织中S100A11和Beclin1蛋白的表达.使用病理图像分析仪分析S100A11和Beclin1蛋白表达在3种组织中的阳性信号灰度值.结果:S100A11蛋白在胃癌组织及胃癌前病变组织中表达的灰度值分别为132.9209±5.6490和133.6706±5.8348,两者均明显低于慢性非萎缩性胃炎中的灰度值138.048±3.5902,差异均有统计学意义(P<0.01),胃癌与胃癌前病变组织中S100A11蛋白表达比较差异无统计学意义;S100A11蛋白的表达与胃癌的分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05),与肿瘤的部位、大小无关.Beclin1蛋白在胃癌组织中表达的灰度值为140.9705±6.2019,明显高于胃癌前病变(136.711±5.5759)和慢性非萎缩性胃炎(130.8024±2.5363),三者两两比较,差异均有统计学意义(P<0.05);Beclin1的表达与胃癌的分化程度及淋巴结转移有关(P<0.05),与肿瘤的部位、大小、浸润深度及TNM分期无关.S100A11和Beclin1在胃癌组织中的表达呈负相关(r=-0.156,P<0.05).结论:S100A11在胃癌组织中高表达,Beclin1在胃癌组织中低表达,S100A11和Beclin1与胃癌的部分生物学行为关系密切,胃癌组织中S100A11和Beclin1的表达呈负相关,提示S100A11和Beclin1在胃组织中的表达失衡,可能是胃癌发生的分子生物学机制之一.
AIM： To investigate the clinical significance of expression of $100All and Beclinl in gastric car- cinoma. METHODS： The expression of $100All and Beclinl proteins were determined usin8 the streptavidin-peroxidase immunohistochemical method in 50 cases of gastric carcinoma, 30 cases of precancerous lesions, and 20 cases of chronic non-atrophic gastritis. RESULTS： The expression level of $100All was significantly lower in gastric carcinoma and pre- cancerous lesions than in chronic non-atrophic gastritis （132.9209±5.6490, 133.6706 ±5.8348 vs138.0480 ±3.5902, both P 〈 0.05）, but there was no significant difference between gastric carci- noma and precancerous lesions. Expression of $100All was significantly correlated with tumor ~rade, infiltration depth, lymph node metastasis,and TNM stage （all P 〈 0.05）, but not with tumor position or size. The expression level of Beclinl was significantly higher in gastric carcinoma （140.9705±6.2019） than in precancerous lesions （136.7110±5.5759） and chronic non-atrophic gastritis （130.8024 ± 2.5363）, and in precancerous lesions than in chronic non-atrophic gastritis （all P 〈 0.05）. Expression of Beclinl was significantly correlated with tumor grade and lymph node metastasis （both P 〈 0.05）, but not with tumor location, size, infiltration depth, or TNM stage. There is a negative correlation between expres- sion of S100All and that of Beclinl in gastric carcinoma （r = -0.156, P 〈 0.05）. CONCLUSION： S100All is lowly and Beclinl is highly expressed in gastric carcinoma. The expression of S100All and Beclinl is closely re- lated with the biological behavior of gastric car- cinoma. There is a negative correlation between expression of s100All and that of Beclinl in gastric carcinoma, indicating that the unbalance of S100All and Beclinl expression is a possible molecular biological mechanism behind the de- velopment of gastric carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第33期3266-3271,共6页
World Chinese Journal of Digestology
