摘要
目的观察盐酸氨基葡萄糖对兔关节腔积血造成的软骨损伤的保护作用。方法成年新西兰大白兔50只,随机分为A、B、C、D、E共5组,每组10只。A、B、C、D组采用自体静脉血4 mL右侧关节腔内注射建立关节腔积血模型。建模后,A、B、C组分别给予25、82.5、500 mg/kg的盐酸氨基葡萄糖灌胃,1次/d;D组为阳性对照组,以6 mL/kg蒸馏水灌胃,1次/d;E组为阴性对照组,以6 mL/kg蒸馏水灌胃,1次/d。灌胃8周后取材。肉眼大体观察,光镜下进行Mankin评分及沙红O染色观察,评估软骨损伤程度;采用免疫组织化学法测定Ⅱ型胶原蛋白(COLⅡ)表达情况。结果软骨大体观察和沙红O染色组织学观察显示,A、B、C组与D组、E组之间差异均有统计学意义(P<0.05)。免疫组织化学提示,A、B、C组COLⅡ表达明显高于D组(P<0.05)。结论盐酸氨基葡萄糖对兔关节腔积血导致的软骨损伤有一定的保护治疗作用,在防治关节腔积血导致的软骨损伤的发生发展方面,具有一定的作用。
Objective To observe the protective effect of glucosamine on cartilage injury caused by rabbit hemarthrosis.Methods Fifty adult New Zealand rabbits were randomly divided into five groups（10 in each group）.A hemarthrosis model was established by injection through venous blood articular cavity.Lavage was conducted with different drug dosages.The positive control group was lavaged with same dosages of distilled water.Lavage was conducted once every day,and materials were collected after 8 weeks.Injury degrees were evaluated by gross observation and by Mankin score and Safrain-O staining under light microscopy.Immunohistochemical techniques were applied to detect the expressions of COLⅡ.Results Gross cartilage observation and histological observation showed significant differences（P0.05） between high-,medium-and low-dosage groups and the positive control group.The immunohistochemical results indicated that COLⅡ expression was significantly higher（P0.05） in each treatment group than that in the positive control group.Conclusions Glucosamine has certain protective effect on blood induced cartilage injury.Glucosamine plays an important role in prevention and treatment of blood induced cartilage injury.
出处
《华西医学》
CAS
2012年第12期1788-1791,共4页
West China Medical Journal
关键词
氨基葡萄糖
关节腔积血
软骨细胞
兔
Glucosamine hedrochloride
Hemarthrosis
Chondrocyte
Rabbit
