摘要
为深入进行胶质瘤细胞凋亡的分了生物学研究及提高胶质瘤辅助化疗疗效打下基础。通过光镜，电镜，荧光显微镜分析，DNA断裂分析及流式细胞仪分析，进行顺铂诱导C6胶质瘤细胞调亡研究。本研究证实在3μg／mLCDDP作用72h，C6细胞出观细胞凋亡；光镜和电镜可见细胞形态学上出现细胞皱缩，染色质浓集贴达；流式细胞仅结果提示有凋亡峰出现，凋亡细胞占细胞总数17.3％±1.2％；荧光显微镜观察出现染色质浓集，染色质断裂：DNA电泳未表现出DNA呈梯状带型断裂。上述结果提示用顺铂成功地诱导大鼠C6胶质瘤细胞发生凋亡。
It will be helpful to explore the molecuIar mechanism of glioma cell apoptosis and improve the effect ofchemotherapy for glioma. Rat C6 glioma cell line was exposed in vilro to cisplatin. Cell apoptosis was ascertained by(1) cell morphology, (2) DNA fragment analysis; (3) fluoroscopy; (4) flow cytometric study. When C6 glioma cellswere treated with cisplatin at 3μg/mL for 72 hours, cell apoptosis occurred. Typical apoptotic morphological features included cell shrinkage and condensation and margination of nuclear chromatin were showed by light and electronmicroscopy. Condensed nuclear chromatin and the fragment of nuclei were demonstrated by fluoroscopy. Apoptoticpeak were identified by flow cytometric study. The apoptotic cells accounted for 17.3% ±1 .2% of the cell population.DNA ladder was not showed by DNA fragment analysis. The apoptosis of C6 glioma was successfully induced bycisplatin.
出处
《基础医学与临床》
CSCD
2000年第3期80-83,共4页
Basic and Clinical Medicine
