摘要
目的 探讨TRAPPCR ELISA间接定量法测定鼻咽癌 (NPC)中端粒酶 (TLMA)的表达。方法 采用TRAPPCRELISA法测定了 18例NPC组织、4例癌旁组织、4例慢性鼻咽炎组织及 2 2个NPCHNE1细胞株、5例白血病骨髓细胞及 2例正常骨髓细胞、6个血管平滑肌细胞株中TLMA的表达。并测定了不同浓度HNE1细胞株及NPC灭活标本中的TLMA活性。结果 NPC活检组织、癌旁组织有较高的TLMA表达 ,而慢性鼻咽炎组织为阴性 (P <0 0 1)。NPCHNE1细胞株、白血病骨髓细胞中亦有较高的TLMA表达。在 10 2 HNE1细胞株中亦能检测到TLMA活性。而 2种正常对照细胞为阴性。灭活后NPC标本中TLMA全部为阴性。结论 无论在NPC体内、体外试验中均有较高的TLMA表达。该定量法特异性好、灵敏度可达 10 2 细胞 ,具有易出结果、可靠、准确。
Objective To detect the expression of telomerase with TRAP PCR ELISA indirect quantitative method in nasopharyngeal carcinoma(NPC).Methods Telomerase activity was detected in 18 cases of NPC,4 cases of the epithelium adjacent to NPC and 4 cases of chronic nasopharyngitis with TRAP PCR ELISA indirect quantitative method.At the same time,the activity of telomerase in 22 HNE 1 cell strains,5 cases of Leukemia marrow cells,2 cases of normal marrow cells and 6 vasculor smooth muscle cell strains as well as in the HNE 1 cell strains of different concentration and NPC after hot inactivating was also explored.Results The telomerase activity in NPC and the epithelium adjacent to NPC reached at 1 03±0 88U and 1 0±0 59U respectively compared with 0 16±0 06U in chronic nasopharyngitis ( P <0 01).There also was a higher telomerase activity in HNE 1 cell strains and leukemia marrow cells than in normal marrow cells and vascular smooth muscle cell strains ( P <0 01).Experiments showed that the telomerase activity of NPC after hot inactivating was lost and detection of the telomerase activity could also be got at a concentration as small as 1×10 2HNE 1 cells/ml.Conclusion There is a high telomerase activity in NPC in vivo and in vitro.TRAP PCR ELISA indirect quantitative assay has many advantages such as high sensitivity,specificity and reliability and is able to reveal and compare different activity status of the telomerase.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第3期207-209,共3页
Cancer Research on Prevention and Treatment
基金
湖南省科委基金
国家自然科学基金!(NO 98ssy10 0 8(2 ) NO 39770 933)
