摘要
目的观察慢性乙型肝炎(CHB)患者外周血淋巴细胞亚群的变化及其与吲哚胺2,3-二氧化酶(IDO)的相关性,探索CHB免疫低下的机制。方法采用随机对照方法,采集50例CHB患者及健康对照组外周静脉血3ml,分别用流式细胞仪检查其CD4+和CD8+T细胞,用荧光实时PCR检测IDO mRNA,比较两组淋巴细胞、IDO的差异及其相关性分析。结果 CHB患者外周血CD4+和CD8+T细胞均较对照组减少,尤以CD4+T细胞为甚;IDO mRNA较对照组明显增多,其与CD4+T细胞及CD4+/CD8+均呈负相关(r=-0.622和0.426,P<0.05)。结论 CHB患者细胞免疫功能低下,IDO的过度表达可能是其主要机制之一。
Objective To observe the correlation between T lymphocyte subsets and the expression of indoleamine-2,3-dioxide enzyme（IDO） so as to explore the role of IDO in immune suppression of chronic hepatitis B（CHB）. Methods The peripheral venous blood samples were taken from 50 CHB patients and 50 healthy people as control group at random and then T lymphocyte subsets and IDO mRNA were detected using FCM and real-time PCR respectively.The difference of T lymphocyte subsets and IDO mRNA were compared between two groups and the correlations of T lymphocyte subsets and IDO mRNA in CHB patients were statistically analyzed. Results In CHB patients,the IDO mRNA was significantly more and CD4＋,CD8＋T lymphocyte as well as ratio of CD4＋/CD8＋ were all less than those in control group（P0.05）.Additionally,IDO mRNA0 was negative correlated with CD4＋ T cells（r=-0.622,P0.05） and ratio of CD4＋/CD8＋（r=-0.426,P0.05）. Conclusion CHB patients have a weakened cellular immune function.IDO over-expression is probably responsible for the immunosuppression in CHB.
出处
《临床军医杂志》
CAS
2012年第6期1290-1293,共4页
Clinical Journal of Medical Officers
基金
中国博士后科学基金(No.20060390678)
