摘要
目的 探讨 EGF、TGFα、TGFβ1、PDGF、b FGF对肺泡 型细胞增生的影响。 方法 在原代培养的成年大鼠肺泡 型细胞中 ,加入不同种类、不同剂量的生长因子 ,作用 48h,测定 3H- Td R掺入、细胞数量和 Ki- 6 7标记指数。 结果 EGF、TGFα均促进肺泡 型细胞 3H - Td R掺入量、细胞数量及 Ki- 6 7阳性标记率增加 ,TGFα比EGF的作用更强 ;TGFβ1的作用与 EGF、TGFα相反。 EGF、TGFα和 TGFβ1对肺泡 型细胞增生的影响均呈明显量效关系。 PDGF和 b FGF对肺泡 型细胞增生的影响未显示出明确的量效规律。同时或先后加入 TGFα和TGFβ1,肺泡 型细胞的增生反应不同。 结论 EGF、TGFα和 TGFβ1对培养的成年大鼠肺泡 型细胞增生有明显的调控作用 ;TGFα和 TGFβ1共同作用于细胞时 ,其生物效应与加入不同因子的时序有关。
Objective\ To study in vitro effects of epidermal growth factor(EGF),transforming growth factor α and β1(TGFα,TGFβ1),platelet derived growth factor(PDGF),and basic fibroblast growth factor(bFGF) on proliferation of alveolar type Ⅱ cells isolated from adult rat.\ Methods\ 3H\|TdR incorporation,cell counting,and Ki\|67 labeling index were used in this experiment.\ Results\ Both EGF and TGFα increased 3H\|TdR incorporation,cell numbers,and Ki\|67 labeling index as compared with the control.TGFα effect was stronger than that of EGF.The effect of TGFβ1 was contrary with EGF and TGFα.Effects of EGF,TGFα,and TGFβ1 on alveolar type Ⅱ cells were dose\|dependent,but there was no consistent dose\|dependent response to either PDGF or bFGF.Cooperative effects of TGFα and TGFβ1 revealed that when TGFα and TGFβ1 were added simultaneously,or TGFα was added prior to TGFβ1.TGFα did not express the stimulating effect as used alone,but the inhibiting effect of TGF β1 was demonstrated.On the contrary,when TGFβ1 was added first,then addition of TGFα exhibited significantly stimulative effect.\ Conclusion\ Special growth factors including EGF,TGFα,and TGFβ1 have been implicated in the regulation of proliferation of cultured alveolar type Ⅱ cells isolated from adult rat.
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第2期152-156,共5页
Acta Anatomica Sinica
基金
国家自然科学基金!( 3 940 0 0 48)
关键词
肺损伤
EGF
TGFΑ
细胞增生
Epidermal growth factor
Transforming growth factor alpha
Transforming growth factor beta
Platelet derived growth factor
Basic fibroblast growth factor
Alveolar type Ⅱ cell
Rat
