摘要
目的 探讨免疫荧光技术对眼瘢痕性类天疱疮 (OCP)的实验诊断意义。方法 应用直接免疫荧光 (DIF)检测 16例OCP患者的结膜和应用间接免疫荧光 (IIF)以鼠食道和正常人结膜为基质检测 34例OCP患者的血清。结果 DIF证实 6例结膜上皮细胞基底膜上有免疫复合物 (IgG和 /或IgA)和 /或C3呈线状沉积。以结膜为基质的IIF证实血清中抗基底膜 (BMZ)抗体 4例 ,抗细胞间物质 (IIS)抗体 14例 ,总阳性率为 5 2 9% ,而以鼠食道为基质的IIF阳性率为38 2 %。结论 DIF对诊断眼瘢痕性类天疱疮是有用的 ;IIF也有助于眼瘢痕性类天疱疮的诊断及疗效评估 ;孤立的上皮内自身免疫瘢痕性结膜炎可能为一新的类型。
Objective To study the usefulness of immunofluorescent technique for the diagnosis of ocularcicatricialpemphigoid . Methods Thirty four patients with ocularcicatricial pemphigoid were studied by in directimmun of luorescence (IIF) on the irserawithrat esophagusandnormalhumanconjunctivaastissuesubstrates .Directimmun of luorescence (DIF) was performed on the conjunctivalbiopsy specimensof 16 patients . Results In 6 casesofthe 16 patients ,DIF of conjunctivashowedalineardeposition of immunoreactants (IgGand/orIgA)and/or C3 on the basement membranez one (BMZ) .Of the 34patients ,4h addetectablecirculatinganti BMZ antibodies ,14and 7patientsdem on stratedantibodiesbinding to the intraepithelia lintercellul arsubstance (IIS) of humanconjunctivaand ratesophagus ,respectively .The positiverate of IIF on humanconjunctivais 529% and on ratesophagus 38 2 % . Conclusions DIF isusefulforthediagnosisofocularcicatricialpemphigoid .IIF also canhelp for the diagnosis and the evaluation of the treatment of ocular cicatricial pemphigoid .Isolated intraepithelial autoimmune fibrozing conjunctivitis may be a new entity .
出处
《眼科研究》
CSCD
2000年第2期123-125,共3页
Chinese Ophthalmic Research
关键词
免疫荧光法
眼瘢痕性
类天疱疮
immunofluorescence ocular cicatricial pemphigoid
