摘要
目的 :观察一氧化氮 (NO)含量含量在葡萄膜炎房水及玻璃体中的变化及 L -硝基精氨酸甲酯 (NG- nitro- L - argininemethyl ester,L- NAME)的治疗效果。 方法 :采用兔眼玻璃体内注射内毒素脂多糖 (L PS)制备葡萄膜炎模型 ,以检测房水蛋白浓度、细胞数目和组织病理观察判定炎症程度 ,同时检测房水及玻璃体中 NO的变化 ,考察 L- NAME在不同时期使用的疗效。 结果 :葡萄膜炎房水及玻璃体中 NO含量显著增高 ;即刻使用 L- NAME,显著降低了 NO水平 ,同时其蛋白浓度、细胞数目及组织炎症程度明显降低 ;6 h使用 L - NAME,则 NO浓度下降不显著 ,蛋白浓度、细胞数目及组织炎症程度没有明显改善。结论 :NO参与了葡萄膜炎的病理过程 ,即刻使用 L - NAME可抑制实验性葡萄膜炎的发展。
Objective: To evaluate the change of nitric oxide (NO) in the aqueous and vitreous humors in uveitis. Methods: Uveitis was induced in rabbits with injection of lipopolysaccharide(LPS) into vitreous cavity. Ocular inflammation was evaluated by measuring protein concentration and leukocyte number in the aqueous humors and by histopathologic examination. NO production concentration was measured in the same time. The effects was evaluated when L NAME was used at various times. Results: NO production level in aqueous and vitreous humor had increased significantly in uveitis; it decreased significantly when L NAME was used at 0 h. Protein concentration and leukocyte numbers and the inflammatory degree decreased significantly at the same time. It decreased slightly when L NAME was used at 6 h. Conclusion: NO takes part in the pathogenesis of uveitis. The development of uveitis is inhibited when L NAME is used at 0 h.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第3期281-283,共3页
Academic Journal of Second Military Medical University
