摘要
以啤酒废酵母为原料,首先筛选出酵母抽提酶与β-葡聚糖酶进行复合酶解,并通过单因素实验初步确定了复合酶解工艺条件;然后通过响应面实验对复合酶解工艺条件进行优化,确定了酶解的最佳工艺条件为:酶解温度50.32℃,初始pH值为5.98,复合酶配比为0.94∶1,复合酶的添加量1.2%,悬浮液浓度10%,NaCl添加量2%,酶解时间24h。水解度模型预测值为73.39%,实测值为73.84%,证明所得回归模型拟合较好。
Using beer waste yeast as a raw material, enzymatic hydrolysis was firstly taken with the complex of selected enzyme of yeast extract and β-glucanase and the enzymatic conditions were primarily obtained though single factor experiments. The optimum hydrolysis conditions were obtained by response surface experiments, which were as follows, hydrolysis temperature of 50.32℃, initial pH 5.98, the composition ratio of enzymes 0.94：1, the addition of complex enzymes 1.2%, concentration of suspension 10%, and the addition of NaCl 2%, hydrolysis time 24h. The model predicted value was 73.39% and the measured was 73.84%, which was fitted well with regression model.
出处
《中国酿造》
CAS
2012年第12期67-71,共5页
China Brewing
基金
江苏高校优势学科建设工程资助项目
关键词
啤酒废酵母
复合酶
氨基酸
水解度
响应面
beer waste yeast
compound enzymes
amino acid
degree of hydrolysis
response surface
