摘要
在以玉米浆为有机氮源,葡萄糖结晶废糖液为碳源的发酵培养基上对大肠杆菌工程菌HBUT-L进行了4L发酵产L-乳酸研究,确定了HBUT-L在玉米浆发酵培养基中能生长,并且可高效快速地将葡萄糖发酵转化成L-乳酸。乳酸产量达到108.3g/L,50h内发酵结束,L-乳酸光学纯度在99%以上,糖酸转化率在97%以上,产率为2.16g(/L.h),是无机盐发酵培养基产率的79.7%。为HBUT-L利用玉米浆发酵产L-乳酸,降低生产成本及其工业化提供参考。
The production of L-lactic acid by engineering Escherichia coli HBUT-L was studied in 4L fermentor using corn steep liquor as organic ni- trogen source and waste sugary liquor of glucose crystallization as carbon source. It was found that HBUT-L could grow and convert glucose into L-lactic acid quickly and efficiently. Maximum lactic acid concentrations of 108.3g/L were obtained; the fermentation time was 50h and the optical purity of L-lactic acid was above 99%. The conversion ratio of glucose to acid was above 97% and the yield was 2.16g/ （L·h）, which was 79.7% of that of inorganic salts fermentation medium. It provides a reference for production of L-lactic acid by HBUT-L using corn steep liquor in industrial scale with reducing production costs.
出处
《中国酿造》
CAS
2012年第12期64-67,共4页
China Brewing
基金
湖北省科技厅科研项目(2011CDA008
2011CDB076)
湖北省教育厅优秀中青年人才项目(Q20121405)
湖北省楚天学者专项基金
关键词
大肠杆菌工程菌
玉米浆
发酵
L-乳酸
engineering Escherichia coli
corn steep liquor
fermentation
L-lactic acid
