摘要
目的以血清药理学方法观察凉血活血中药对人脐静脉内皮细胞(ECV304)增殖的影响,从体外实验角度研究,为临床对新生血管类眼病的治疗寻找相应的干预靶点.方法设立空白血清组对照应用MTT法检测不同浓度的凉血活血含药血清对血管内皮细胞ECV304细胞毒性考察及增殖影响,并检测VEGF含量的变化.结果 20%浓度凉血活血含药血清组既可抑制细胞增殖又对细胞无毒性作用.结论凉血活血中药抑制血管新生可能与其通过抑制ECV304细胞增殖及降低VEGF的表达有关.
Objective To study the therapeutic effect liangxuehuoxue traditional Chinese medicines on the supression of ECV304 by serum pharmacological method,and to explore the interventional target for treatment of neovessels related eye diseases.Methods ECV304cells were cultured with medicated serum with different concentrations of liangxuehuoxue medicines.The proliferation rate of endothelial cells was assayed with MTT methods,the level of VEGF was tested.Result Medicated serum with liangxuehuoxue medicines at concentration of 20% could inhibit the proliferation of ECV304 cells and the expression of VEGF.Conclusion Liangxuehuoxue traditional Chinese medicines cna inhibit the ECV304 cells proliferation and the expression of VEGF,and this may be related to inhibition of neo vessels.
出处
《昆明医科大学学报》
CAS
2012年第11期38-41,52,共5页
Journal of Kunming Medical University
基金
云南省科技厅自然科学研究基金资助项目(2011FZ257)
