摘要
目的:分析张家口地区汉族人乙型肝炎病毒基因分型与耐药变异。方法:分别采用巢式荧光实时定量PCR及荧光实时定量PCR检测张家口地区98例汉族乙肝病毒感染患者基因型及耐药变异,所有患者接受多拉米定抗病毒治疗。结果:张家口地区98例汉族人乙型肝炎病毒基因型主要以B型,C型,B+C混合型为主,其中B型29例(29.59%),C型30例(30.61%),B+C混合型39例(39.80%);发生YMDD变异总变异率为58.16%(57/98)。在发生YMDD变异者中,YVDD变异占61.40%(35/57),YIDD变异占38.60%(22/57)。基因型为B型者YMDD变异率为21.43%(21/98),C型者为33.67%(33/98),B+C混合型者为44.90%(44/98),三者之间差异有统计学意义(P<0.05)。结论:张家口地区汉族人乙型肝炎病毒基因型主要为B型,C型与B+C混合型;B+C混合型患者使用拉米夫定后发生YMDD变异率最高。
Objective： To analyze Hepatitis B virus （HBV） genotypes and drug resistance mutations among people in Zhangjiakou. Methods： The genotypes and drug resistant mutation of HBV in 98 infected cases of Han nationality in Zhangjiakou were detected by nested quantitative real-time PCR and real-time fluorescent PCR. Results： The main genotypes among the 98 cases in Zhangiiakou were genotype B （29.59%）, C （30.61%） and B＋C （39.80%）. The total mutation rate of YMDD was 58.16% （57/98）, among which, YVDD mutation accounted for 61.40% （35/57）, and YIDD mutation was 38.60% （22/57）. The YMDD mutation rate in genotype B was 21.43% （21/98）, C was 33.67% （33/98） and B＋C was 44.90% （44/98） respectively, and the difference among them was statistically significant （P〈0.05）. Conclusion： The main HBV genotypes of Han nationality in Zhangjiakou are genotypes B, C and B＋C, and the genotype B＋C has the highest YMDD mutation rate after Lamivudine treatment.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第12期1550-1553,共4页
China Journal of General Surgery
