摘要
目的观察人参皂甙Rgl对大鼠海马神经元缺糖氧／复糖氧后谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）的影响。方法海马神经元培养8～10d，随机分为正常对照组、模型组、人参皂甙Rgl低、中、高剂量组（5gmol／L，20gmol／L，60gmol／L）。建立大鼠海马神经元缺糖氧／复糖氧模型，复糖氧后6h以生物化学法观察各组海马神经元GSH含量和GPx活性的变化；复糖氧后24h以Hochest染色法检测细胞凋亡，并检测各组海马神经元四甲基偶氮唑盐（MTT）代谢率。结果与模型组相比，人参皂甙Rgl中、高剂量组海马神经元GSH含量、GPx活性显著升高，凋亡显著减少，MTT代谢率显著提高（P〈0．001），人参皂甙Rgl低剂量组变化不明显（P〉0．05）。结论人参皂甙Rgl可通过提高缺糖氧神经元GSH含量和GPx活性，发挥脑保护作用。
Objective To explore the effect of Ginsenoside Rgl on glutathion （GSH） level and glutathion peroxidase （GPx） activity after oxygen-glucose deprivation/reperfusion in cultured hippocampal cells. Methods The model of oxygen-glucose deprivation and reperfusion were established with the hippocampal neurons of rats. They were randomly divided into control group, model group and Ginsenoside Rgl treatment groups （5 μmol/L, 20 μmol/L, 60 μmol/L）. The GSH level and GPx activity were measured 6 h after reperfusion. The apoptosis and the metabolic rate of methyl thiazolyl tetrazolium （MTT） were detected 24 h after repcrfusion. Results Compared with model group, the GSH level, GPx activity, and metabolic rate of MTT improved （P〈0.001）, and the apoptosis decreased in the Ginsenoside Rgl groups （P〈 0.001） except with the dosage of 5 μmol/L （P〉0.05）. Conclusion Ginsenoside Rgl can protect the brain from ischemia by increasing the GSH level and GPx activity.
出处
《中国康复理论与实践》
CSCD
北大核心
2012年第12期1112-1115,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
江苏高校优势学科建设工程项目(Jx10231081)
