摘要
目的:观察凤尾草总黄酮对人骨肉瘤MG-63细胞体外迁移能力的影响,并探讨其作用机制。方法:利用乙醇萃取法制备凤尾草总黄酮,通过划痕实验、Transwell实验观察不同浓度凤尾草总黄酮对同样条件培养下骨肉瘤MG-63细胞迁移能力的影响,并采用Western blot方法检测不同浓度凤尾草总黄酮对MG-63细胞内白介素6(IL-6)蛋白表达水平的影响。结果:同未加药物对照组相比,经高浓度凤尾草总黄酮(5与10 g.L-1)处理后,MG-63细胞的划痕愈合能力明显减弱,穿膜细胞的数量显著减少;同时,细胞内IL-6蛋白的表达水平明显降低,并与凤尾草总黄酮的浓度呈负相关性。结论:凤尾草总黄酮抑制了骨肉瘤MG-63细胞的迁移能力,其作用与抑制MG-63细胞内的IL-6蛋白表达水平有关。
Objective：To investigate the effect of the Pteris multifida Flavones on MG-63 cells metastasis of human osteosarcoma cell line in vitro and to explore the action mechanisms of the Pteris multifida Flavones.Methods：Pteris multifida preparation was made by ehanol,the effect of the different concentrations of Pteris multifida Flavoneson the experimental metastasis ability of MG-63 cells was observed by scratch test and Transwell metastasis experiments.The levels of IL-6 protein in MG-63 cells were detected by Western Blotting.Results：Comparing with control group,the Pteris multifida Flavones（5 and10 g·L-1）inhibited the MG-63 cells wound closure significantly at 24 hours time points and decreased the number of cells passing through filter membrane.The levels of IL-6 protein in MG-63 cells were decreased significantly after treated with the Pteris multifida Flavones.Conclusion：The Pteris multifida Flavones is able to inhibit the metastasis ability of MG-63 cells.The action mechanisms of the Pteris multifida Flavones are related to the inhibition of IL-6 protein levels in MG-63 cells.
出处
《辽宁中医药大学学报》
CAS
2013年第1期42-44,共3页
Journal of Liaoning University of Traditional Chinese Medicine
