摘要
目的:观察中药制剂复方仙草颗粒对IgA肾病防治效果及可能作用机制。方法:采用切除一侧肾脏,反复尾静脉注射较大剂量葡萄球菌肠毒素B(SEB)的方法,制作IgA肾病大鼠实模型。随机分为模型组、福辛普利组[蒙诺冲剂液,含药0.33 g/mL,10 mL/(kg.d)]、复方仙草颗粒(由八仙草、三七、薏苡仁、制大黄、黄芪、甘草组成)小、中、大剂量组[复方仙草颗粒10、20、40 g/(kg.d)],另设正常对照组。疗程8周。检测大鼠24 h尿蛋白定量、尿红细胞计数;血清胱抑素C、血清肌酐;肾组织IgA(免疫荧光)、肾小球系膜细胞数及纤黏蛋白(FN,免疫化学法)表达。结果 (:1)复方仙草颗粒各组及福辛普利组24 h尿蛋白定量、尿红细胞排泄率均较模型组明显改善(P<0.01);与福辛普利组比较,复方仙草颗粒大剂量组尿红细胞改善更显著(P>0.05);复方仙草颗粒中、大剂量组24 h尿蛋白定量与福辛普利组比较,差异无统计学意义(P>0.05)。(2)各药物干预组血清CystatinC与模型组比较,均明显改善(P<0.05,P<0.01);复方仙草颗粒大剂量组血清CystatinC与福辛普利组比较,差异无统计学意义(P>0.05);各组大鼠间SCr差异无统计学意义(P>0.05)。(3)各药物干预组与模型组比较,肾小球系膜细胞数及FN表达均显著降低(P<0.05,P<0.01);复方仙草颗粒中、大剂量组与福辛普利组比较,系膜细胞数差异无统计学意义(P>0.05);复方仙草颗粒大剂量组与福辛普利组比较,FN表达差异亦无统计学意义(P>0.05)。结论:复方仙草颗粒能改善IgA肾病大鼠血尿、蛋白尿,保护肾功能,其作用机制可能与减少IgA在肾组织沉积,抑制肾小球系膜细胞增殖及细胞外基质积聚有关。
Objective：To explore the effect and possible acting mechanism of compound Xiancao granules on treating IgA nephropathy rat model.Methods：The model of IgA nephropathy（IgAN）was made by injecting steophylocdccus enterotoxin B（SEB）via rats caudal vein repeatedly and unilateral nephrectomy.All the IgA nephropathy rats were randomly divided into model group,Fosinopril[0.33 g/mL,10？mL/（kg·d）]group,compound Xiancao granules low[10 g/（kg·d）],middle[20 g/（kg·d）]and high-dose [40 g/（kg·d）] group.Healthy mice were chosen as healthy control group.After gavage with drugs for 8 weeks,laboratory indexes as urine protein,erythrocyte count,serum CystatinC（SCysC）and serum creatinine（Scr）were detected.IgA deposition（immunofluorescence）,mesangial cell count and fibronecin（FN）expression（immunohistochemistry）in renal tissue were observed.Results：Compared with model group,the levels of urine protein and erythrocyte count in each dose group with compound Xiancao granules were markedly improved（P0.01）.SCysC in each dose group with compound Xiancao granules were significantly lower than that in model group（P0.05,P0.01）.Mesangial cell count and FN expression in each dose group with compound Xiancao granules was significantly decreased（P0.05,P0.01）.Conclusion：Compound Xiancao granules can ameliorate the renal injury in experimental IgA nephropathy.The mechanism may be related to reducing IgA deposition in renal tissue,inhibiting mesangial cell proliferation and extracellular matrix accumulation.
出处
《辽宁中医药大学学报》
CAS
2013年第1期39-41,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
广西壮族自治区自然科学基金资助项目(0991171)
广西科学研究与技术开发项目(0719006-3-6)
