摘要
本文应用抗人结肠癌单克隆抗体MC_3对32例结肠腺病性息肉。11例绒毛状腺病,18例结肠腺瘤伴不典型增生和36例结肠腺癌组织进行了PAP免疫组织化学染色,并同时采用醛复红—阿辛蓝(AF/AB)复合染色法,观察了结肠癌异型粘液分泌与MC_3表达的关系。结果表明,MC_3主要为结肠肿瘤的标记癌组织MC_3表达有三种类型即腺腔型腔缘型和细胞型、前两型表达亦见于结肠腺瘤,其中腺瘸性息肉和绒毛状腺瘤MC_3表达的阳性率存在差异。本文还观察到癌组织MC_3表达的类型与强度癌细胞分泌非硫酸粘液有一定关系。 本文提示MC_3与结肠肿瘤细胞的分泌有关,并可将其用于结肠癌细胞生物学特性研究及结肠肿瘤的辅助诊断。
The expression of monoclonal antibody MC3 and its relationship to abno-ormal mucin secretion were investigated in colon tumors, including polypoid adenomas (32 cases ), villous adenomas (11), adenomas with atypical hyperplasia(18) and adenocarcino-mas(36), with PAP immunohistochemistry and aldehyde fuscin-alcian blue histochemical methods. The results showed that MC3, which mainly appeared in adenocarcinoma of colon, expressed luminal .marginal and cellular types in adenoepithelium. The former two types could be seen also in adenoma of colon, but different positive staining of MC3 was exised. Some connection between expression of MC3 and adnormal mucin secretion of colon could be observed too. It is suggested that the appearance of MC3 may be related to this secretion of mucin.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1991年第4期302-304,共3页
Chinese Journal of Cancer
关键词
结肠肿瘤
粘液反应
单克隆抗体
colorectal adenocarcinoma
mucinous reaction
monoclonal antibody
AF/ AB (Aldehyde Fuchsin-Alcian Bluue)
