摘要
本实验采用人癌细胞体外培养的方法,证明了天佛参口服液(HRD)可抑制人癌细胞集落的形成,直接杀伤癌细胞,抑制人癌细胞的生长,降低其生长率,生长率和药物浓度呈负相关。对MGC_(803)、SMMC_(7721)、Hela细胞的半数抑制浓度(IC_(50))分别为10.42μl/ml 12.55μl/ml和9.30μl/ml。显微分光光度计检测MGC_(803)细胞小鼠S_(180)腹水细胞核DNA显示:低浓度时使细胞相对DNA含量直方图明显右移,说明低浓度时即可影响癌细胞的代谢,阻滞细胞增殖于G_2+M期,高浓度时虽有右移,但不明显说明高浓度时的主要机制是直接杀伤作用。
The method of cancer cells cultioation in vitro has been used to show that HRD (Tianfosheng decoction) can inhibnt colont formation of human cancer cells, inhibit their growth or kill them directly. The growth rate showed negative correlation with logarithmic concentration of HRD. The 50% lingibitory concentration (IC 50) for M-GC803, SMMC7721 and HeLa cells were 10.42, 12.55 and 9.30/μg/ml, respectively. It has been shown that the histogram of DNA content shifts to the right obviously in low concentration group, but not in high concentration group, through the measurement of DNA in MGC803 and S- 180 ascitic cells by computerized microspectrophotometre. The results showed that in low concentration HRD can suppress the growth of cancer cells with cells accumulated at G2+M phase, and in high concentration the main action is cell killing directly.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1991年第3期190-192,197,共4页
Chinese Journal of Cancer
