摘要
目的分析医院不同科室来源的耐甲氧西林金黄色葡萄球菌(MRSA)菌株基因分型同源性,为控制MRSA医院感染流行提供科学依据。方法使用细菌基因组重复序列聚合酶链反应(REP-PCR)以及高级微生物基因分型系统(DiversiLab)细菌同源性分析技术对23株医院感染的MRSA进行基因分型。结果 23株MRSA分为4个基因型,A型、D型主要分布于急诊观察室,B型、C型主要分布于中医外科。结论中医外科存在以B型、C型基因型为流行株的MRSA医院感染爆发;REP-PCR技术和DiversiLab自动化细菌同源性分型技术可成为医院感染病原研究的有效手段。
Objective To provide scientific reference for controlling hospital acquired infection through analyzing the genotype homology of methicillin-resistant Staphylococcus aureus （MRSA） which were isolated from different departments of hospital. Methods The genotypes of 23 strains of hospital acquired MRSA were analyzed by repetitive polymerase chain reaction （REP-PCR） and DiversiLab genotype system. Results Hospital acquired MRSA were classified into 4 kinds of genotypes（ A, B, C and D）. A and D types were mainly distributed in Emergency Observation Room, while B and C types were mainly distributed in Traditional Chinese Medicine Surgery Department. Conclusions B and C types may cause infection outbreak in Traditional Chinese Medicine Surgery Department as epidemic strains. REP-PCR andDiversiLab genotype system can be efficient ways for research of pathogen on nosocomial infection.
出处
《检验医学》
CAS
2012年第12期1031-1034,共4页
Laboratory Medicine
关键词
耐甲氧西林金黄色葡萄球菌
医院感染
基因分型
Methicillin-resistant Staphylococcus aureus
Nosocomial infection
Genotyping
